N-Glycosylation Site Analysis of Proteins from Saccharomyces cerevisiae by Using Hydrophilic Interaction Liquid Chromatography-Based Enrichment, Parallel Deglycosylation, and Mass Spectrometry

	N-Glycosylation Site Analysis of Proteins from Saccharomyces cerevisiae by Using Hydrophilic Interaction Liquid Chromatography-Based Enrichment, Parallel Deglycosylation, and Mass Spectrometry
	Cao, Liwei; Yu, Long; Guo, Zhimou; Shen, Aijin; Guo, Yunu; Liang, Xinmiao
刊名	journal of proteome research
	2014-03-01
卷号	13 期号:3 页码:1485-1493
关键词	glycoproteomics Saccharomyces cerevisiae hydrophilic interaction liquid chromatography glycopeptide mass spectrometry yeast
ISSN号	1535-3893
通讯作者	于龙 ; 梁鑫淼
产权排序	待补充
合作状况	英
英文摘要	n-glycosylation site analysis of baker's yeast saccharomyces cerevisiae is of fundamental significance to elucidate the molecular mechanism of human congenital disorders of glycosylation (cdg). here we present a mass spectrometry (ms)-based workflow for the profiling of n-glycosylated sites in s. cerevisiae proteins. in this workflow, proteolytic glycopeptides were enriched by using a hydrophilic material named click te-cys to improve the glycopeptide selectivity and coverage. to enhance the reliability of the identified results, the enriched glycopeptides were subjected to parallel deglycosylation by using two endoglycosidases (i.e., pngase f and endo h-f), respectively, prior to lc-ms/ms analysis. on the basis of the workflow, a total of 135 n-glycosylated sites including 6 known, 93 potential, and 36 novel sites were identified and mapped to 79 proteins. among the novel-type sites, nine sites from eight proteins, which were simultaneously identified via pngase f and endo h-f deglycosylation, are believed to possess high confidence. the established workflow, together with the profile of n-glycosylated sites, will contribute to the improvement of s. cerevisiae model for revealing the pathogenesis of cdg.
学科主题	物理化学
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biochemical research methods
研究领域[WOS]	biochemistry & molecular biology
关键词[WOS]	congenital disorders ; linked glycoproteins ; glycopeptide-enrichment ; selective enrichment ; serum glycoproteins ; identification ; yeast ; matrix ; lectin ; quantification
收录类别	SCI
资助信息	1，1
原文出处	1493
语种	英语
WOS记录号	WOS:000332756300028
公开日期	2014-09-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/119718]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Cao, Liwei,Yu, Long,Guo, Zhimou,et al. N-Glycosylation Site Analysis of Proteins from Saccharomyces cerevisiae by Using Hydrophilic Interaction Liquid Chromatography-Based Enrichment, Parallel Deglycosylation, and Mass Spectrometry[J]. journal of proteome research,2014,13(3):1485-1493.
APA	Cao, Liwei,Yu, Long,Guo, Zhimou,Shen, Aijin,Guo, Yunu,&Liang, Xinmiao.(2014).N-Glycosylation Site Analysis of Proteins from Saccharomyces cerevisiae by Using Hydrophilic Interaction Liquid Chromatography-Based Enrichment, Parallel Deglycosylation, and Mass Spectrometry.journal of proteome research,13(3),1485-1493.
MLA	Cao, Liwei,et al."N-Glycosylation Site Analysis of Proteins from Saccharomyces cerevisiae by Using Hydrophilic Interaction Liquid Chromatography-Based Enrichment, Parallel Deglycosylation, and Mass Spectrometry".journal of proteome research 13.3(2014):1485-1493.