One-pot synthesis of magnetic colloidal nanocrystal clusters coated with chitosan for selective enrichment of glycopeptides

	One-pot synthesis of magnetic colloidal nanocrystal clusters coated with chitosan for selective enrichment of glycopeptides
	Fang, Chunli 1; Xiong, Zhichao 2; Qin, Hongqiang 3; Huang, Guang 3; Liu, Jing 3; Ye, Mingliang 3; Feng, Shun 1; Zou, Hanfa 3
刊名	analytica chimica acta
	2014-09-02
卷号	841 页码:99-105
关键词	Glycopeptides Magnetic colloidal nanocrystal clusters Chitosan Hydrophilic interaction liquid chromatography
ISSN号	0003-2670
通讯作者	封顺 ; 邹汉法
产权排序	待补充
合作状况	英
英文摘要	selective enrichment of glycopeptides prior to the mass spectrometry (ms) analysis is essential due to ion suppression effect during ionization caused by the co-presence of non-glycosylated peptides. among the enrichment approaches, hydrophilic interaction liquid chromatography (hilic) based on magnetic separation has become a popular method in recent years. as the conventional synthesis procedures of these materials are tedious and time-consuming with at least four steps. herein, magnetic colloidal nanocrystal clusters coated with chitosan (fe3o4@cs mcncs) have been successfully prepared by a simple one-pot method. the resulting fe3o4@cs mcncs demonstrated an excellent ability for glycopeptide enrichment with high selectivity, low detection limit and high binding capacity. furthermore, in the analysis of real complicated biological sample, 283 unique n-glycosylation sites corresponding to 175 glycosylated proteins were identified in three replicate analyses of 45 mu g protein sample extracted from hela cells, indicating the great potential in detection and identification of low abundant glycopeptides in glycoproteome analysis. (c) 2014 elsevier b.v. all rights reserved.
学科主题	物理化学
WOS标题词	science & technology ; physical sciences
类目[WOS]	chemistry, analytical
研究领域[WOS]	chemistry
关键词[WOS]	interaction liquid-chromatography ; mass-spectrometry ; glycosylation ; nanoparticles ; polysaccharide ; identification ; silica
收录类别	SCI
资助信息	3,3
原文出处	105
语种	英语
WOS记录号	WOS:000339990900013
公开日期	2014-09-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/119694]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Xinjiang Univ, Coll Chem & Chem Engn, Minist Educ Xinjiang Uyghur Autonomous Reg, Key Lab Oil Gas Fine Chem, Urumqi 830046, Peoples R China 2.E China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China 3.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China
推荐引用方式 GB/T 7714	Fang, Chunli,Xiong, Zhichao,Qin, Hongqiang,et al. One-pot synthesis of magnetic colloidal nanocrystal clusters coated with chitosan for selective enrichment of glycopeptides[J]. analytica chimica acta,2014,841:99-105.
APA	Fang, Chunli.,Xiong, Zhichao.,Qin, Hongqiang.,Huang, Guang.,Liu, Jing.,...&Zou, Hanfa.(2014).One-pot synthesis of magnetic colloidal nanocrystal clusters coated with chitosan for selective enrichment of glycopeptides.analytica chimica acta,841,99-105.
MLA	Fang, Chunli,et al."One-pot synthesis of magnetic colloidal nanocrystal clusters coated with chitosan for selective enrichment of glycopeptides".analytica chimica acta 841(2014):99-105.