Improvement of mannitol production by Lactobacillus brevis mutant 3-A5 based on dual-stage pH control and fed-batch fermentations

	Improvement of mannitol production by Lactobacillus brevis mutant 3-A5 based on dual-stage pH control and fed-batch fermentations
	Yue, Min 1,2; Cao, Hailong 1; Zhang, Jianping 1,2; Li, Shuguang 1; Meng, Yanyu 1,2; Chen, Wei 1; Huang, Lishuxin 1,2; Du, Yuguang 1
刊名	world journal of microbiology & biotechnology
	2013-10-01
卷号	29 期号:10 页码:1923-1930
关键词	Mannitol Dual-stage pH control Lactobacillus brevis Batch fermentation Fed-batch fermentation
ISSN号	0959-3993
通讯作者	杜昱光
产权排序	待补充
合作状况	英
英文摘要	lactobacillus brevis 3-a5 was isolated and expected to produce mannitol efficiently by regulating ph in batch and fed-batch fermentations. in 48 h batch fermentations with free and constant ph, the optimal ph for cell growth and mannitol production in the first 24 h of incubation was 5.5, whereas that for mannitol production in the second 24 h of incubation was 4.5. to achieve high cell density and mannitol yield simultaneously, a dual-stage ph control strategy was proposed based on the kinetic analysis of mannitol production. the ph value was controlled at 5.5 for the first 12 h of fermentation and subsequently shifted to 4.5 until the fermentation was completed. under dual-stage ph control fermentation, a 103 g/l yield of mannitol with a volumetric production rate of 3.7 g/l/h was achieved after 28 h. the dual-stage ph control fed-batch fermentation strategy was further developed to improve mannitol yield, wherein the yield increased by 109 % to 215 g/l after 98 h of fermentation. this value is the highest yield of mannitol ever reported using l. brevis.
学科主题	物理化学
WOS标题词	science & technology ; life sciences & biomedicine
类目[WOS]	biotechnology & applied microbiology
研究领域[WOS]	biotechnology & applied microbiology
关键词[WOS]	intermedius nrrl b-3693 ; lactic-acid bacteria ; reuteri crl 1101 ; organic-acids ; growth
收录类别	SCI
资助信息	1，1
原文出处	1930
语种	英语
WOS记录号	WOS:000325035100020
公开日期	2014-09-11
内容类型	期刊论文
源URL	[http://159.226.238.44/handle/321008/119287]
专题	大连化学物理研究所_中国科学院大连化学物理研究所
作者单位	1.Chinese Acad Sci, Nat Prod & Glycobiotechnol Res Grp, Liaoning Prov Key Lab Carbohydrates, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Yue, Min,Cao, Hailong,Zhang, Jianping,et al. Improvement of mannitol production by Lactobacillus brevis mutant 3-A5 based on dual-stage pH control and fed-batch fermentations[J]. world journal of microbiology & biotechnology,2013,29(10):1923-1930.
APA	Yue, Min.,Cao, Hailong.,Zhang, Jianping.,Li, Shuguang.,Meng, Yanyu.,...&Du, Yuguang.(2013).Improvement of mannitol production by Lactobacillus brevis mutant 3-A5 based on dual-stage pH control and fed-batch fermentations.world journal of microbiology & biotechnology,29(10),1923-1930.
MLA	Yue, Min,et al."Improvement of mannitol production by Lactobacillus brevis mutant 3-A5 based on dual-stage pH control and fed-batch fermentations".world journal of microbiology & biotechnology 29.10(2013):1923-1930.