Enhanced Humoral and Cell-Mediated Immune Responses Generated by Cationic Polymer-Coated PLA Microspheres with Adsorbed HBsAg

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	Enhanced Humoral and Cell-Mediated Immune Responses Generated by Cationic Polymer-Coated PLA Microspheres with Adsorbed HBsAg
	Chen, Xiaoming 1,2; Liu, Yuying 3; Wang, Lianyan 1; Liu, Yuan 1,2; Zhang, Weifeng 1,2; Fan, Bei 4; Ma, Xiaowei 4; Yuan, Qipeng 3; Ma, Guanghui 1; Su, Zhiguo 1
刊名	MOLECULAR PHARMACEUTICS
	2014-06-01
卷号	11 期号:6 页码:1772-1784
关键词	PLA microsphere cationic polymer HBsAg adsorption Th1
ISSN号	1543-8384
其他题名	Mol. Pharm.
中文摘要	Surface-engineered particulate delivery systems for vaccine administration have been widely investigated in experimental and clinical studies. However, little is known about charge-coated microspheres as potential recombinant subunit protein antigen delivery systems in terms of adsorption and related immune responses. In the present study, cationic polymers, including chitosan (CS), chitosan chloride (CSC), and polyethylenimine (PEI), were used to coat PIA microspheres to build positively charged surfaces. Antigen adsorption capacity was enhanced with increased surface charge of coated microspheres. In macrophages, HBsAg adsorbed on the surface of cationic microspheres specifically enhanced antigen uptake and augmented CD86, MHC I, and MHC II expression and IL-1 beta, IL-6, TNF-alpha, and IL-12 release. Antigens were more likely to localize independent of lysosomes after phagocytosis in antigen-attached cationic microsphere formulations. After intraperitoneal immunization, cationic microsphere-based vaccine formulations generated a rapid and efficient humoral immune response and cytokine release as compared with aluminum-adsorbed vaccine and free antigens in vivo. Moreover, microspheres coated with cationic polymers with relatively high positive charges and higher antigen adsorption exhibited strong stimulation of the Th1 response. In conclusion, PLA microspheres coated with cationic polymers may be a potential recombinant antigen delivery system to induce strong cell and humoral immune responses.
英文摘要	Surface-engineered particulate delivery systems for vaccine administration have been widely investigated in experimental and clinical studies. However, little is known about charge-coated microspheres as potential recombinant subunit protein antigen delivery systems in terms of adsorption and related immune responses. In the present study, cationic polymers, including chitosan (CS), chitosan chloride (CSC), and polyethylenimine (PEI), were used to coat PIA microspheres to build positively charged surfaces. Antigen adsorption capacity was enhanced with increased surface charge of coated microspheres. In macrophages, HBsAg adsorbed on the surface of cationic microspheres specifically enhanced antigen uptake and augmented CD86, MHC I, and MHC II expression and IL-1 beta, IL-6, TNF-alpha, and IL-12 release. Antigens were more likely to localize independent of lysosomes after phagocytosis in antigen-attached cationic microsphere formulations. After intraperitoneal immunization, cationic microsphere-based vaccine formulations generated a rapid and efficient humoral immune response and cytokine release as compared with aluminum-adsorbed vaccine and free antigens in vivo. Moreover, microspheres coated with cationic polymers with relatively high positive charges and higher antigen adsorption exhibited strong stimulation of the Th1 response. In conclusion, PLA microspheres coated with cationic polymers may be a potential recombinant antigen delivery system to induce strong cell and humoral immune responses.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Medicine, Research & Experimental ; Pharmacology & Pharmacy
研究领域[WOS]	Research & Experimental Medicine ; Pharmacology & Pharmacy
关键词[WOS]	PLASMACYTOID DENDRITIC CELLS ; B-VIRUS INFECTION ; VACCINE ADJUVANT ; EXOGENOUS ANTIGENS ; ALUMINUM ADJUVANTS ; CROSS-PRESENTATION ; DNA VACCINES ; HIV-1 P24 ; T-CELLS ; DELIVERY
收录类别	SCI
原文出处	://WOS:000336941900005
语种	英语
WOS记录号	WOS:000336941900005
公开日期	2014-08-28
内容类型	期刊论文
版本	出版稿
源URL	[http://ir.ipe.ac.cn/handle/122111/10889]
专题	过程工程研究所_研究所（批量导入）
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Natl Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Chinese Acad Sci, Graduated Univ, Beijing 100049, Peoples R China 3.Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing 100029, Peoples R China 4.Hualan Biol Engn Inc, Xinxiang 453003, Henan, Peoples R China
推荐引用方式 GB/T 7714	Chen, Xiaoming,Liu, Yuying,Wang, Lianyan,et al. Enhanced Humoral and Cell-Mediated Immune Responses Generated by Cationic Polymer-Coated PLA Microspheres with Adsorbed HBsAg[J]. MOLECULAR PHARMACEUTICS,2014,11(6):1772-1784.
APA	Chen, Xiaoming.,Liu, Yuying.,Wang, Lianyan.,Liu, Yuan.,Zhang, Weifeng.,...&Su, Zhiguo.(2014).Enhanced Humoral and Cell-Mediated Immune Responses Generated by Cationic Polymer-Coated PLA Microspheres with Adsorbed HBsAg.MOLECULAR PHARMACEUTICS,11(6),1772-1784.
MLA	Chen, Xiaoming,et al."Enhanced Humoral and Cell-Mediated Immune Responses Generated by Cationic Polymer-Coated PLA Microspheres with Adsorbed HBsAg".MOLECULAR PHARMACEUTICS 11.6(2014):1772-1784.