A conditional lethal mutation in Rana grylio virus ORF 53R resulted in a marked reduction in virion formation

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	A conditional lethal mutation in Rana grylio virus ORF 53R resulted in a marked reduction in virion formation
	He, Li-Bo; Gao, Xiao-Chan; Ke, Fei; Zhang, Qi-Ya
刊名	VIRUS RESEARCH
	2013-11-06
卷号	177 期号:2 页码:194-200
关键词	Conditional lethal mutant Rana grylio virus (RGV) Envelope protein 53R Recombinant iridovirus lac repressor/operator system Gene expression
ISSN号	0168-1702
通讯作者	Zhang, QY (reprint author), Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China.
中文摘要	Rana grylio virus (RGV) is a pathogenic iridovirus that has resulted in high mortality in cultured frog. Here, a recombinant RGV (i53R-RGV-lacIO) containing the inducible lac repressor! operator system was constructed. i53R-RGV-lacIO was a conditional lethal mutant in which the expression of envelope protein 53R was regulated by IPTG. i53R-RGV-lacIO shared characteristics similar to RGV in the presence of IPTG. However, the expression level of 53R, the ability of plaques formation, and the virus titers were strongly reduced in the absence of IPTG. Electron microscopy showed that the number of progeny virus produced by i53R-RGV-lacIO was remarkably reduced without IPTG. Furthermore, over-expression of 53R in vitro could increase titers of i53R-RGV-lacIO in the absence of IPTG. Therefore, the current data suggested that the lac repressor/operator system could regulate gene expression in the recombinant iridovirus. Our study was thought to be the first report of the system in aquatic virus. (C) 2013 Elsevier B.V. All rights reserved.
英文摘要	Rana grylio virus (RGV) is a pathogenic iridovirus that has resulted in high mortality in cultured frog. Here, a recombinant RGV (i53R-RGV-lacIO) containing the inducible lac repressor! operator system was constructed. i53R-RGV-lacIO was a conditional lethal mutant in which the expression of envelope protein 53R was regulated by IPTG. i53R-RGV-lacIO shared characteristics similar to RGV in the presence of IPTG. However, the expression level of 53R, the ability of plaques formation, and the virus titers were strongly reduced in the absence of IPTG. Electron microscopy showed that the number of progeny virus produced by i53R-RGV-lacIO was remarkably reduced without IPTG. Furthermore, over-expression of 53R in vitro could increase titers of i53R-RGV-lacIO in the absence of IPTG. Therefore, the current data suggested that the lac repressor/operator system could regulate gene expression in the recombinant iridovirus. Our study was thought to be the first report of the system in aquatic virus. (C) 2013 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Virology
研究领域[WOS]	Virology
关键词[WOS]	GENE-EXPRESSION ; VACCINIA VIRUS ; FISH CELLS ; FAMILY-IRIDOVIRIDAE ; MEMBRANE-PROTEIN ; LCDV-C ; VECTOR ; GENOME ; MORPHOGENESIS ; REPLICATION
收录类别	SCI
资助信息	National Major Basic Research Program [2010CB126303, 2009CB118704]; National Natural Science Foundation of China [31072239, 31270213]; Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-EW-Z-3]; FEBL research grant [2011FBZ12, 2008FBZ16]
语种	英语
WOS记录号	WOS:000328443800010
公开日期	2014-08-06
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/19976]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	He, Li-Bo,Gao, Xiao-Chan,Ke, Fei,et al. A conditional lethal mutation in Rana grylio virus ORF 53R resulted in a marked reduction in virion formation[J]. VIRUS RESEARCH,2013,177(2):194-200.
APA	He, Li-Bo,Gao, Xiao-Chan,Ke, Fei,&Zhang, Qi-Ya.(2013).A conditional lethal mutation in Rana grylio virus ORF 53R resulted in a marked reduction in virion formation.VIRUS RESEARCH,177(2),194-200.
MLA	He, Li-Bo,et al."A conditional lethal mutation in Rana grylio virus ORF 53R resulted in a marked reduction in virion formation".VIRUS RESEARCH 177.2(2013):194-200.