Impedimetric biosensor based on cell-mediated bioimprinted films for bacterial detection

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	Impedimetric biosensor based on cell-mediated bioimprinted films for bacterial detection
	Qi, Peng1,2 ; Wan, Yi 1,2; Zhang, Dun 1
刊名	BIOSENSORS & BIOELECTRONICS
	2013-01-15
卷号	39 期号:1 页码:282-288
关键词	Sulfate-reducing bacteria Graphene Electrochemical impedance spectroscopy Bioimprinted film Electrodeposition
ISSN号	0956-5663
通讯作者	Zhang, D
中文摘要	This work presents the synthesis of bacteria-mediated bioimprinted films for selective bacterial detection. Marine pathogen sulfate-reducing bacteria (SRB) were chosen as the template bacteria. Chitosan (CS) doped with reduced graphene sheets (RGSs) was electrodeposited on an indium tin oxide electrode, and the resulting RGSs-CS hybrid film served as a platform for bacterial attachment. The electrodeposition conditions were optimized to obtain RGSs-CS hybrid films with excellent electrochemical performance. A layer of nonconductive CS film was deposited to embed the pathogen, and acetone was used to wash away the bacterial templates. Electrochemical impedance spectroscopy was performed to characterize the stepwise modification process and monitor the SRB population. Faradic impedance measurements revealed that the charge transfer resistance (R-ct) increased with increased SRB concentration. A linear relationship between Delta R-ct and the logarithm of SRB concentration was obtained within the concentration range of 1.0 x 10(4) cfu mL(-1) to 1.0 x 10(8) cfu mL(-1). The impedimetric sensor showed good selectivity towards SRB based on size and shape. Hence, selectivity for bacterial detection can be improved if the bioimprinting technique is combined with other bio-recognition elements. (C) 2012 Elsevier B.V. All rights reserved.
英文摘要	This work presents the synthesis of bacteria-mediated bioimprinted films for selective bacterial detection. Marine pathogen sulfate-reducing bacteria (SRB) were chosen as the template bacteria. Chitosan (CS) doped with reduced graphene sheets (RGSs) was electrodeposited on an indium tin oxide electrode, and the resulting RGSs-CS hybrid film served as a platform for bacterial attachment. The electrodeposition conditions were optimized to obtain RGSs-CS hybrid films with excellent electrochemical performance. A layer of nonconductive CS film was deposited to embed the pathogen, and acetone was used to wash away the bacterial templates. Electrochemical impedance spectroscopy was performed to characterize the stepwise modification process and monitor the SRB population. Faradic impedance measurements revealed that the charge transfer resistance (R-ct) increased with increased SRB concentration. A linear relationship between Delta R-ct and the logarithm of SRB concentration was obtained within the concentration range of 1.0 x 10(4) cfu mL(-1) to 1.0 x 10(8) cfu mL(-1). The impedimetric sensor showed good selectivity towards SRB based on size and shape. Hence, selectivity for bacterial detection can be improved if the bioimprinting technique is combined with other bio-recognition elements. (C) 2012 Elsevier B.V. All rights reserved.
学科主题	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
研究领域[WOS]	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
关键词[WOS]	SULFATE-REDUCING BACTERIA ; MOLECULARLY IMPRINTED POLYMERS ; GRAPHENE SHEETS ; NANOPARTICLES ; SURFACE ; HYBRIDIZATION ; FLUORESCENCE ; VANCOMYCIN ; PATHOGENS ; ELECTRODE
收录类别	SCI
原文出处	10.1016/j.bios.2012.07.078
语种	英语
WOS记录号	WOS:000309486300046
公开日期	2014-07-17
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/16372]
专题	海洋研究所_海洋腐蚀与防护研究发展中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Shandong Prov Key Lab Corros Sci, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Qi, Peng,Wan, Yi,Zhang, Dun. Impedimetric biosensor based on cell-mediated bioimprinted films for bacterial detection[J]. BIOSENSORS & BIOELECTRONICS,2013,39(1):282-288.
APA	Qi, Peng,Wan, Yi,&Zhang, Dun.(2013).Impedimetric biosensor based on cell-mediated bioimprinted films for bacterial detection.BIOSENSORS & BIOELECTRONICS,39(1),282-288.
MLA	Qi, Peng,et al."Impedimetric biosensor based on cell-mediated bioimprinted films for bacterial detection".BIOSENSORS & BIOELECTRONICS 39.1(2013):282-288.