Proteomic Analysis in Nitrogen-Deprived Isochrysis galbana during Lipid Accumulation

	Proteomic Analysis in Nitrogen-Deprived Isochrysis galbana during Lipid Accumulation
	Song, Pingping 1,2; Li, Ling 1; Liu, Jianguo 1
刊名	PLOS ONE
	2013-12-05
卷号	8 期号:12
ISSN号	1932-6203
通讯作者	Song, PP
中文摘要	The differentially co-expressed proteins in N-deprived and N-enriched I. galbana were comparatively analyzed by using two dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization-time-of-flight/time-of-flight-mass spectrometry (MALDI-TOF/TOF-MS) with the aim of better understanding lipid metabolism in this oleaginous microalga. Forty-five of the 900 protein spots showed dramatic changes in N-deprived I. galbana compared with the N-enriched cells. Of these, 36 protein spots were analyzed and 27 proteins were successfully identified. The identified proteins were classified into seven groups by their molecular functions, including the proteins related to energy production and transformation, substance metabolism, signal transduction, molecular chaperone, transcription and translation, immune defense and cytoskeleton. These altered proteins slowed cell growth and photosynthesis of I. galbana directly or indirectly, but at the same time increased lipid accumulation. Eight key enzymes involved in lipid metabolism via different pathways were identified as glyceraldehyde-3-phosphate dehydrogenase (GAPDH), phosphoglycerate kinase (PGK), enolase, aspartate aminotransferase (AST), fumarate hydratase (FH), citrate synthase (CS), O-acetyl-serine lyase (OAS-L) and ATP sulfurylase (ATPS). The results suggested that the glycolytic pathway and citrate transport system might be the main routes for lipid anabolism in N-deprived I. galbana, and that the tricarboxylic acid (TCA) cycle, glyoxylate cycle and sulfur assimilation system might be the major pathways involved in lipid catabolism.
英文摘要	The differentially co-expressed proteins in N-deprived and N-enriched I. galbana were comparatively analyzed by using two dimensional electrophoresis (2-DE) and matrix-assisted laser desorption/ionization-time-of-flight/time-of-flight-mass spectrometry (MALDI-TOF/TOF-MS) with the aim of better understanding lipid metabolism in this oleaginous microalga. Forty-five of the 900 protein spots showed dramatic changes in N-deprived I. galbana compared with the N-enriched cells. Of these, 36 protein spots were analyzed and 27 proteins were successfully identified. The identified proteins were classified into seven groups by their molecular functions, including the proteins related to energy production and transformation, substance metabolism, signal transduction, molecular chaperone, transcription and translation, immune defense and cytoskeleton. These altered proteins slowed cell growth and photosynthesis of I. galbana directly or indirectly, but at the same time increased lipid accumulation. Eight key enzymes involved in lipid metabolism via different pathways were identified as glyceraldehyde-3-phosphate dehydrogenase (GAPDH), phosphoglycerate kinase (PGK), enolase, aspartate aminotransferase (AST), fumarate hydratase (FH), citrate synthase (CS), O-acetyl-serine lyase (OAS-L) and ATP sulfurylase (ATPS). The results suggested that the glycolytic pathway and citrate transport system might be the main routes for lipid anabolism in N-deprived I. galbana, and that the tricarboxylic acid (TCA) cycle, glyoxylate cycle and sulfur assimilation system might be the major pathways involved in lipid catabolism.
学科主题	Science & Technology - Other Topics
WOS标题词	Science & Technology
类目[WOS]	Multidisciplinary Sciences
研究领域[WOS]	Science & Technology - Other Topics
关键词[WOS]	SEED OIL CONTENT ; CYCLOTELLA-CRYPTICA ; GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE ; THALASSIOSIRA-PSEUDONANA ; CHEMICAL-COMPOSITION ; OVER-EXPRESSION ; ATP SULFURYLASE ; HIGHER-PLANTS ; GROWTH ; PROTEIN
收录类别	SCI
原文出处	10.1371/journal.pone.0082188
语种	英语
WOS记录号	WOS:000328566100091
公开日期	2014-07-17
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/16715]
专题	海洋研究所_海洋生物技术研发中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Song, Pingping,Li, Ling,Liu, Jianguo. Proteomic Analysis in Nitrogen-Deprived Isochrysis galbana during Lipid Accumulation[J]. PLOS ONE,2013,8(12).
APA	Song, Pingping,Li, Ling,&Liu, Jianguo.(2013).Proteomic Analysis in Nitrogen-Deprived Isochrysis galbana during Lipid Accumulation.PLOS ONE,8(12).
MLA	Song, Pingping,et al."Proteomic Analysis in Nitrogen-Deprived Isochrysis galbana during Lipid Accumulation".PLOS ONE 8.12(2013).