Preparation, characterization and antioxidant activity of two partially N-acetylated chitotrioses

	Preparation, characterization and antioxidant activity of two partially N-acetylated chitotrioses
	Li, Kecheng 1,2; Liu, Song 1; Xing, Ronge1 ; Qin, Yukun 1; Li, Pengcheng1
刊名	CARBOHYDRATE POLYMERS
	2013-02-15
卷号	92 期号:2 页码:1730-1736
关键词	Chitotriose N-acetylchitotriose N Separation Antioxidant activity N '-diacetylchitotriose
ISSN号	0144-8617
通讯作者	Xing, RG
中文摘要	The preparation of chitooligosaccharides with single degree of polymerization and well-defined degree of acetylation is of significance to identify the components possessing strong biofunction. This study focuses on the preparation of two partially acetylated chitotrioses (N-acetylchitotriose and N,N'-diacetylchitotriose), involving a two-step process: (i) partially N-acetylation of chitotriose and (ii) separation of different acetylated chitotrioses. The chitotriose was N-acetylated and characterized with FT-IR and ESI-MS. N-acetylchitotriose and N,N'-diacetylchitotriose were separated from the acetylated chitotriose mixture by CM Sepharose Fast Flow. Subsequently, the antioxidant activities of two partially acetylated chitotrioses and original chitotriose were investigated, including hydroxyl radical and superoxide radical scavenging activity. The results showed that the activities of the three oligomers were in a dose-dependence manner and related to their degree of acetylation. The N,N'-diacetylchitotriose with high degree of acetylation exhibited the highest antioxidant activity. (C) 2012 Elsevier Ltd. All rights reserved.
英文摘要	The preparation of chitooligosaccharides with single degree of polymerization and well-defined degree of acetylation is of significance to identify the components possessing strong biofunction. This study focuses on the preparation of two partially acetylated chitotrioses (N-acetylchitotriose and N,N'-diacetylchitotriose), involving a two-step process: (i) partially N-acetylation of chitotriose and (ii) separation of different acetylated chitotrioses. The chitotriose was N-acetylated and characterized with FT-IR and ESI-MS. N-acetylchitotriose and N,N'-diacetylchitotriose were separated from the acetylated chitotriose mixture by CM Sepharose Fast Flow. Subsequently, the antioxidant activities of two partially acetylated chitotrioses and original chitotriose were investigated, including hydroxyl radical and superoxide radical scavenging activity. The results showed that the activities of the three oligomers were in a dose-dependence manner and related to their degree of acetylation. The N,N'-diacetylchitotriose with high degree of acetylation exhibited the highest antioxidant activity. (C) 2012 Elsevier Ltd. All rights reserved.
学科主题	Chemistry ; Polymer Science
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Applied ; Chemistry, Organic ; Polymer Science
研究领域[WOS]	Chemistry ; Polymer Science
关键词[WOS]	BIOLOGICAL-ACTIVITIES ; SCAVENGING ACTIVITY ; CHITIN DEACETYLASE ; IN-VITRO ; CHITOSAN ; CHITOOLIGOSACCHARIDES ; OLIGOSACCHARIDES ; OLIGOMERS ; CHITIN/CHITOSAN ; HYDROLYSIS
收录类别	SCI
原文出处	10.1016/j.carbpol.2012.11.028
语种	英语
WOS记录号	WOS:000315616900099
公开日期	2014-07-17
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/16714]
专题	海洋研究所_海洋生物技术研发中心
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Li, Kecheng,Liu, Song,Xing, Ronge,et al. Preparation, characterization and antioxidant activity of two partially N-acetylated chitotrioses[J]. CARBOHYDRATE POLYMERS,2013,92(2):1730-1736.
APA	Li, Kecheng,Liu, Song,Xing, Ronge,Qin, Yukun,&Li, Pengcheng.(2013).Preparation, characterization and antioxidant activity of two partially N-acetylated chitotrioses.CARBOHYDRATE POLYMERS,92(2),1730-1736.
MLA	Li, Kecheng,et al."Preparation, characterization and antioxidant activity of two partially N-acetylated chitotrioses".CARBOHYDRATE POLYMERS 92.2(2013):1730-1736.