Spatio-temporal Variations in the Molecular Diversity of Microeukaryotes in Particular Ciliates in Soil of the Yellow River Delta, China

CORC > 海洋研究所 > 中国科学院海洋研究所 > 海洋生物分类与系统演化实验室

	Spatio-temporal Variations in the Molecular Diversity of Microeukaryotes in Particular Ciliates in Soil of the Yellow River Delta, China
	Zhao, Feng 1,2; Xu, Kuidong1 ; Zhang, Dechao 1; Xu, KD
刊名	JOURNAL OF EUKARYOTIC MICROBIOLOGY
	2013-05-01
卷号	60 期号:3 页码:282-290
关键词	Ciliate community coastal wetland microeukaryotic diversity salinization seasonal variation
ISSN号	1066-5234
通讯作者	Xu, KD
中文摘要	The Yellow River delta in China is one of the most active regions of landocean interaction. It has suffered serious salinization due to drying-up of the Yellow River, rising sea level, and seashore erosion, and thus represents a special and extreme environment. We evaluated the microeukaryotic molecular diversity and its response to change of seasons and environmental variables, in particular salinity in the soil of the Yellow River delta, by denaturing gradient gel electrophoresis (DGGE) and gene sequencing. The sequencing of the microeukaryotic DGGE bands revealed the presence of diverse groups dominated by protists in particular ciliates. We further recovered a high diversity of marine and soil ciliates inhabiting in coastal soil using the ciliate-specific DGGE. The neighbor-joining tree indicated that the ciliate 18S rDNA sequences from high-salinity soil were affiliated to Colpodea, Spirotrichea, Litostomatea, and Oligohymenophorea, while all the sequences unique to the low-salinity soil were affiliated to Colpodea. Statistical analysis indicated that the microeukaryotic molecular diversity was significantly different among sites, while statistically indistinguishable among seasons. Soil salinity might be the main factor regulating the distribution of microeukaryotes in the soil from the Yellow River delta.
英文摘要	The Yellow River delta in China is one of the most active regions of landocean interaction. It has suffered serious salinization due to drying-up of the Yellow River, rising sea level, and seashore erosion, and thus represents a special and extreme environment. We evaluated the microeukaryotic molecular diversity and its response to change of seasons and environmental variables, in particular salinity in the soil of the Yellow River delta, by denaturing gradient gel electrophoresis (DGGE) and gene sequencing. The sequencing of the microeukaryotic DGGE bands revealed the presence of diverse groups dominated by protists in particular ciliates. We further recovered a high diversity of marine and soil ciliates inhabiting in coastal soil using the ciliate-specific DGGE. The neighbor-joining tree indicated that the ciliate 18S rDNA sequences from high-salinity soil were affiliated to Colpodea, Spirotrichea, Litostomatea, and Oligohymenophorea, while all the sequences unique to the low-salinity soil were affiliated to Colpodea. Statistical analysis indicated that the microeukaryotic molecular diversity was significantly different among sites, while statistically indistinguishable among seasons. Soil salinity might be the main factor regulating the distribution of microeukaryotes in the soil from the Yellow River delta.
学科主题	Microbiology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Microbiology
研究领域[WOS]	Microbiology
关键词[WOS]	GRADIENT GEL-ELECTROPHORESIS ; EUKARYOTIC DIVERSITY ; COMMUNITY STRUCTURE ; ECOSYSTEM ; PATTERNS ; PROTOZOA ; DNA ; MIGRATION ; PROTISTS ; RECOVERY
收录类别	SCI
原文出处	10.1111/jeu.12035
语种	英语
WOS记录号	WOS:000318245900006
公开日期	2014-07-17
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/16343]
专题	海洋研究所_海洋生物分类与系统演化实验室
通讯作者	Xu, KD
作者单位	1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Feng,Xu, Kuidong,Zhang, Dechao,et al. Spatio-temporal Variations in the Molecular Diversity of Microeukaryotes in Particular Ciliates in Soil of the Yellow River Delta, China[J]. JOURNAL OF EUKARYOTIC MICROBIOLOGY,2013,60(3):282-290.
APA	Zhao, Feng,Xu, Kuidong,Zhang, Dechao,&Xu, KD.(2013).Spatio-temporal Variations in the Molecular Diversity of Microeukaryotes in Particular Ciliates in Soil of the Yellow River Delta, China.JOURNAL OF EUKARYOTIC MICROBIOLOGY,60(3),282-290.
MLA	Zhao, Feng,et al."Spatio-temporal Variations in the Molecular Diversity of Microeukaryotes in Particular Ciliates in Soil of the Yellow River Delta, China".JOURNAL OF EUKARYOTIC MICROBIOLOGY 60.3(2013):282-290.