Influences of external nutrient conditions on the transcript levels of a nitrate transporter gene in Skeletonema costatum

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	Influences of external nutrient conditions on the transcript levels of a nitrate transporter gene in Skeletonema costatum
	Liu Yun 1,2; Song Xiuxian1 ; Han Xiaotian 1; Yu Zhiming1 ; Yu, ZM
刊名	ACTA OCEANOLOGICA SINICA
	2013-06-01
卷号	32 期号:6 页码:82-88
关键词	high-affinity nitrate transporter gene phosphate starvation nitrate concentrations real-time PCR Skeletonema costatum
ISSN号	0253-505X
通讯作者	Yu, ZM
中文摘要	To verify the feasibility of high-affinity nitrate transporter gene (Nrt2) as an indicator of nitrogen status, changes in the transcript levels of transcripts associated with phosphate starvation and different nitrate concentrations were studied using real-time quantitative reverse-transcription PCR (QRT-PCR) technology in batch cultures of Skeletonema costatum. The results show that compared with P-replete condition, P starvation could reduce the Nrt2 transcript levels apparently. Nrt2 transcript levels had a significant negative linear correlation with nitrate concentrations below 40 mu mol/L. The results of 48 h short-term incubation experiment under different nitrate concentrations confirmed this correlation, and the following regression equation is built: y = -3.305x + 98.95, R-2 = 0.988, where x represents nitrate concentrations (<40 mu mol/L) and y represents the Nrt2 transcript levels.
英文摘要	To verify the feasibility of high-affinity nitrate transporter gene (Nrt2) as an indicator of nitrogen status, changes in the transcript levels of transcripts associated with phosphate starvation and different nitrate concentrations were studied using real-time quantitative reverse-transcription PCR (QRT-PCR) technology in batch cultures of Skeletonema costatum. The results show that compared with P-replete condition, P starvation could reduce the Nrt2 transcript levels apparently. Nrt2 transcript levels had a significant negative linear correlation with nitrate concentrations below 40 mu mol/L. The results of 48 h short-term incubation experiment under different nitrate concentrations confirmed this correlation, and the following regression equation is built: y = -3.305x + 98.95, R-2 = 0.988, where x represents nitrate concentrations (<40 mu mol/L) and y represents the Nrt2 transcript levels.
学科主题	Oceanography
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Oceanography
研究领域[WOS]	Oceanography
关键词[WOS]	THALASSIOSIRA-PSEUDONANA ; MARINE-PHYTOPLANKTON ; NITROGEN DEFICIENCY ; EXPRESSION ; STRESS ; BACILLARIOPHYCEAE ; PRYMNESIOPHYCEAE ; ECOSYSTEMS ; METABOLISM ; CLONING
收录类别	SCI
原文出处	10.1007/s13131-013-0324-2
语种	英语
WOS记录号	WOS:000320094400011
公开日期	2014-07-17
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/16478]
专题	海洋研究所_海洋生态与环境科学重点实验室
通讯作者	Yu, ZM
作者单位	1.Chinese Acad Sci, Key Lab Marine Ecol & Environm Sci, Inst Oceanol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Liu Yun,Song Xiuxian,Han Xiaotian,et al. Influences of external nutrient conditions on the transcript levels of a nitrate transporter gene in Skeletonema costatum[J]. ACTA OCEANOLOGICA SINICA,2013,32(6):82-88.
APA	Liu Yun,Song Xiuxian,Han Xiaotian,Yu Zhiming,&Yu, ZM.(2013).Influences of external nutrient conditions on the transcript levels of a nitrate transporter gene in Skeletonema costatum.ACTA OCEANOLOGICA SINICA,32(6),82-88.
MLA	Liu Yun,et al."Influences of external nutrient conditions on the transcript levels of a nitrate transporter gene in Skeletonema costatum".ACTA OCEANOLOGICA SINICA 32.6(2013):82-88.