The molecular characterization of a cyclophilin A from Chinese mitten crab Eriocheir sinensis and the antifungal activity of its recombinant protein

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	The molecular characterization of a cyclophilin A from Chinese mitten crab Eriocheir sinensis and the antifungal activity of its recombinant protein
	Wang, Mengqiang1 ; Zhang, Daoxiang 1,2; Wang, Lingling 1; Gai, Yunchao 1; Zhou, Zhi 1; Zhang, Huan 1; Song, Linsheng 1
刊名	ELECTRONIC JOURNAL OF BIOTECHNOLOGY
	2013-03-15
卷号	16 期号:2 页码:5
关键词	antifungal activity cyclophilin A Eriocheir sinensis immune response PPIase activity
ISSN号	0717-3458
通讯作者	Song, LS
中文摘要	Background: Cyclophilin A (CypA), a receptor for the immunosuppressive agent cyclosporin A (CsA), is a cis-trans peptidyl-prolyl isomerase (PPIase) which accelerates the cis-trans isomerization of prolyl-peptide bonds and interacts with a variety of proteins to regulate their activities. Results: The full-length cDNA of crab Eriocheir sinensis CypA (EsCypA) was cloned by EST and RACE technique. The complete sequence of EsCypA cDNA contained a 5' untranslated region (UTR) of 50 bp, a 3' UTR of 233 bp with a polyA tail, and an open reading frame (ORF) of 495 bp encoding a polypeptide of 164 amino acids with the predicted molecular weight of 17.36 kDa. The deduced amino acid sequence of EsCypA contained two highly conserved signature sequences of peptidyl-prolyl cis-trans isomerase and a pro-isomerase domain. The mRNA transcripts of EsCypA were detectable in all the examined tissues, including haemocytes, gill, hepatopancreas, gonad, muscle and heart, with higher expression level in hepatopancreas and gonad. No significant difference in the relative mRNA expression level of EsCypA was observed during the whole course of bacteria challenge, whereas it was up-regulated during fungi challenge. The purified recombinant protein rEsCypA exhibited a significant PPIase activity and an antifungal activity. Conclusions: All these results indicated that it was a typical CypA member and potentially involved in the innate immune responses of crab.
英文摘要	Background: Cyclophilin A (CypA), a receptor for the immunosuppressive agent cyclosporin A (CsA), is a cis-trans peptidyl-prolyl isomerase (PPIase) which accelerates the cis-trans isomerization of prolyl-peptide bonds and interacts with a variety of proteins to regulate their activities.
学科主题	Biotechnology & Applied Microbiology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	CIS-TRANS ISOMERASE ; ACTIVATED T-CELLS ; SIGNALING PATHWAYS ; CHLAMYS-FARRERI ; BINDING-PROTEIN ; NUCLEAR FACTOR ; CLONING ; IMMUNOPHILINS ; HIV-1 ; GENE
收录类别	SCI
原文出处	10.2225/vol16-issue2-fulltext-5
语种	英语
WOS记录号	WOS:000318349300006
公开日期	2014-07-17
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/16654]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wang, Mengqiang,Zhang, Daoxiang,Wang, Lingling,et al. The molecular characterization of a cyclophilin A from Chinese mitten crab Eriocheir sinensis and the antifungal activity of its recombinant protein[J]. ELECTRONIC JOURNAL OF BIOTECHNOLOGY,2013,16(2):5.
APA	Wang, Mengqiang.,Zhang, Daoxiang.,Wang, Lingling.,Gai, Yunchao.,Zhou, Zhi.,...&Song, Linsheng.(2013).The molecular characterization of a cyclophilin A from Chinese mitten crab Eriocheir sinensis and the antifungal activity of its recombinant protein.ELECTRONIC JOURNAL OF BIOTECHNOLOGY,16(2),5.
MLA	Wang, Mengqiang,et al."The molecular characterization of a cyclophilin A from Chinese mitten crab Eriocheir sinensis and the antifungal activity of its recombinant protein".ELECTRONIC JOURNAL OF BIOTECHNOLOGY 16.2(2013):5.