The C-reactive protein of tongue sole Cynoglossus semilaevis is an acute phase protein that interacts with bacterial pathogens and stimulates the antibacterial activity of peripheral blood leukocytes

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	The C-reactive protein of tongue sole Cynoglossus semilaevis is an acute phase protein that interacts with bacterial pathogens and stimulates the antibacterial activity of peripheral blood leukocytes
	Li, Mo-fei1,2 ; Chen, Cheng 1,2; Li, Jun 1,3; Sun, Li 1
刊名	FISH & SHELLFISH IMMUNOLOGY
	2013-02-01
卷号	34 期号:2 页码:623-631
关键词	C-reactive protein Pentraxin Cynoglossus semilaevis Phagocytosis
ISSN号	1050-4648
通讯作者	Sun, L
中文摘要	Pentraxins are a family of evolutionarily conserved proteins that play an important part in innate immunity. C-reactive protein (CRP) is a member of the pentraxin family and in humans is known to be the major acute phase protein. In this work, we report the identification and analysis of a CRP, CsCRP, from half-smooth tongue sole (Cynoglossus semilaevis). CsCRP is composed of 228 amino acid residues and possesses a Pentraxin/CRP domain. Expression of CsCRP occurred in a wide range of tissues and was upregulated by pathogen infection in kidney, spleen, blood, and, in particular, liver. Following bacterial infection, CsCRP level in blood rose rapidly within 12 h and was approximately 3.8 fold of that of the basal level. Purified recombinant CsCRP (rCsCRP) was able to interact with Gram-negative and Gram-positive bacteria including those of pathogenic nature in a dose-dependent manner. When peripheral blood leukocytes (PBL) were infected with bacterial pathogen in the presence of rCsCRP, the respiratory burst and phagocytic capacity of the cells were increased to significant extents. Taken together, these results indicate that CsCRP is an acute phase protein that plays a role in innate immune defense against bacterial infection. (C) 2012 Elsevier Ltd. All rights reserved.
英文摘要	Pentraxins are a family of evolutionarily conserved proteins that play an important part in innate immunity. C-reactive protein (CRP) is a member of the pentraxin family and in humans is known to be the major acute phase protein. In this work, we report the identification and analysis of a CRP, CsCRP, from half-smooth tongue sole (Cynoglossus semilaevis). CsCRP is composed of 228 amino acid residues and possesses a Pentraxin/CRP domain. Expression of CsCRP occurred in a wide range of tissues and was upregulated by pathogen infection in kidney, spleen, blood, and, in particular, liver. Following bacterial infection, CsCRP level in blood rose rapidly within 12 h and was approximately 3.8 fold of that of the basal level. Purified recombinant CsCRP (rCsCRP) was able to interact with Gram-negative and Gram-positive bacteria including those of pathogenic nature in a dose-dependent manner. When peripheral blood leukocytes (PBL) were infected with bacterial pathogen in the presence of rCsCRP, the respiratory burst and phagocytic capacity of the cells were increased to significant extents. Taken together, these results indicate that CsCRP is an acute phase protein that plays a role in innate immune defense against bacterial infection. (C) 2012 Elsevier Ltd. All rights reserved.
学科主题	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
研究领域[WOS]	Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
关键词[WOS]	AMYLOID-P-COMPONENT ; PENTRAXIN-LIKE PROTEIN ; CARP CYPRINUS-CARPIO ; CATFISH ICTALURUS-PUNCTATUS ; SNAPPER PAGRUS-AURATUS ; INNATE IMMUNITY ; IMMUNOPROTECTIVE ANALYSIS ; STREPTOCOCCUS-PNEUMONIAE ; PATTERN-RECOGNITION ; INFLAMMATORY AGENTS
收录类别	SCI
原文出处	10.1016/j.fsi.2012.12.001
语种	英语
WOS记录号	WOS:000315076100025
公开日期	2014-07-17
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/16643]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Lake Super State Univ, Sch Biol Sci, Sault Ste Marie, MI USA
推荐引用方式 GB/T 7714	Li, Mo-fei,Chen, Cheng,Li, Jun,et al. The C-reactive protein of tongue sole Cynoglossus semilaevis is an acute phase protein that interacts with bacterial pathogens and stimulates the antibacterial activity of peripheral blood leukocytes[J]. FISH & SHELLFISH IMMUNOLOGY,2013,34(2):623-631.
APA	Li, Mo-fei,Chen, Cheng,Li, Jun,&Sun, Li.(2013).The C-reactive protein of tongue sole Cynoglossus semilaevis is an acute phase protein that interacts with bacterial pathogens and stimulates the antibacterial activity of peripheral blood leukocytes.FISH & SHELLFISH IMMUNOLOGY,34(2),623-631.
MLA	Li, Mo-fei,et al."The C-reactive protein of tongue sole Cynoglossus semilaevis is an acute phase protein that interacts with bacterial pathogens and stimulates the antibacterial activity of peripheral blood leukocytes".FISH & SHELLFISH IMMUNOLOGY 34.2(2013):623-631.