Role of alternative sigma factor 54 (RpoN) from Vibrio anguillarum M3 in protease secretion, exopolysaccharide production, biofilm formation, and virulence

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	Role of alternative sigma factor 54 (RpoN) from Vibrio anguillarum M3 in protease secretion, exopolysaccharide production, biofilm formation, and virulence
	Hao, Bin 1,2; Mo, Zhao-Lan 1,3; Xiao, Peng 1; Pan, Hai-Jian 1,2; Lan, Xin 1,2; Li, Gui-Yang 1,3
刊名	APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
	2013-03-01
卷号	97 期号:6 页码:2575-2585
关键词	Vibrio anguillarum RpoN (sigma(54)) Biofilm Exopolysaccharides Metalloproteases Virulence
ISSN号	0175-7598
通讯作者	Mo, ZL
中文摘要	The sigma factor sigma(54) (RpoN) is an important regulator of bacterial response to environmental stresses. Here, we demonstrate the roles of RpoN in Vibrio anguillarum M3 by comparative investigation of physiological phenotypes and virulence of the wild-type, an rpoN mutant, and an rpoN complemented strain. Disruption of rpoN was found to decrease biofilm formation, production of exopolysaccharides, and production of the metalloproteases EmpA and PrtV. Injection experiments in fish showed that the M3 Delta rpoN mutant was attenuated in virulence when administrated either by intramuscular injection or by immersion challenge. Slower proliferation of the mutant in fish was also observed. Complementation of the mutant strain with rpoN restored some of the phenotypes to wild-type levels. RpoN was involved in regulation of some virulence-associated genes, as shown by real-time quantitative reverse PCR analysis. These results revealed a pleiotropic regulatory role of RpoN in biofilm formation, production of proteases and exopolysaccharides, and virulence in V. anguillarum M3.
英文摘要	The sigma factor sigma(54) (RpoN) is an important regulator of bacterial response to environmental stresses. Here, we demonstrate the roles of RpoN in Vibrio anguillarum M3 by comparative investigation of physiological phenotypes and virulence of the wild-type, an rpoN mutant, and an rpoN complemented strain. Disruption of rpoN was found to decrease biofilm formation, production of exopolysaccharides, and production of the metalloproteases EmpA and PrtV. Injection experiments in fish showed that the M3 Delta rpoN mutant was attenuated in virulence when administrated either by intramuscular injection or by immersion challenge. Slower proliferation of the mutant in fish was also observed. Complementation of the mutant strain with rpoN restored some of the phenotypes to wild-type levels. RpoN was involved in regulation of some virulence-associated genes, as shown by real-time quantitative reverse PCR analysis. These results revealed a pleiotropic regulatory role of RpoN in biofilm formation, production of proteases and exopolysaccharides, and virulence in V. anguillarum M3.
学科主题	Biotechnology & Applied Microbiology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	MEDIATED IRON UPTAKE ; ESCHERICHIA-COLI ; PSEUDOMONAS-AERUGINOSA ; CHEMOTACTIC MOTILITY ; HEMOLYSIN GENE ; UPTAKE SYSTEM ; METALLOPROTEASE ; IDENTIFICATION ; EXPRESSION ; COLONIZATION
收录类别	SCI
原文出处	10.1007/s00253-012-4372-x
语种	英语
WOS记录号	WOS:000315650100027
公开日期	2014-07-17
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/16621]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Hao, Bin,Mo, Zhao-Lan,Xiao, Peng,et al. Role of alternative sigma factor 54 (RpoN) from Vibrio anguillarum M3 in protease secretion, exopolysaccharide production, biofilm formation, and virulence[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2013,97(6):2575-2585.
APA	Hao, Bin,Mo, Zhao-Lan,Xiao, Peng,Pan, Hai-Jian,Lan, Xin,&Li, Gui-Yang.(2013).Role of alternative sigma factor 54 (RpoN) from Vibrio anguillarum M3 in protease secretion, exopolysaccharide production, biofilm formation, and virulence.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,97(6),2575-2585.
MLA	Hao, Bin,et al."Role of alternative sigma factor 54 (RpoN) from Vibrio anguillarum M3 in protease secretion, exopolysaccharide production, biofilm formation, and virulence".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 97.6(2013):2575-2585.