Analysis on the dynamic changes of the amount of WSSV in Chinese shrimp Fenneropenaeus chinensis during infection

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	Analysis on the dynamic changes of the amount of WSSV in Chinese shrimp Fenneropenaeus chinensis during infection
	Sun, Yumiao; Li, Fuhua; Xiang, Jianhai
刊名	AQUACULTURE
	2013-02-15
卷号	376 页码:124-132
关键词	WSSV Viral load Dynamic changes Fenneropenaeus chinensis
ISSN号	0044-8486
通讯作者	Li, FH
中文摘要	White spot syndrome virus (WSSV) is the most severe viral pathogen to the shrimp culture industry worldwide. The temporal and spatial dynamic changes of WSSV in Chinese shrimp Fenneropenaeus chinensis maintained at 22 +/- 1 degrees C during infection via injection were studied through the quantitative real-time PCR method. The pleopods were selected for virus quantification at 0, 6, 12, 18, 24, 27, 30, 33, 36, 39, 42, 45 and 48 hours post-injection (hpi). Besides the pleopod, different tissues including the hepatopancreas, nerve, heart, eyestalk, muscle, lymphoid organ, gut, stomach, gill and skin were also selected for viral load analysis at 0, 12, 27 and 30 hpi as well as the moribund stage after 40 hpi. The curve of viral load change in vivo assumed a very similar shape with the typical viral one-step growth curve. The infection process was classified into three stages: the light infection stage (0 to 18 hpi) when the viral load in the pleopod was at the level of nomore than 10(3)/ng DNA and no mortality appeared; the moderate infection stage (18 to 45 hpi), corresponding to the logarithmic phase of WSSV replication, when the viral load in the pleopod ranged from 10(3) to 10(5)/ng DNA and a low mortality rate was observed; and the heavy infection stage when WSSV propagation went into the plateau phage and massive mortality happened. A turning point from chronic infection to acute infection was suggested from 27 to 30 hpi when the viral load in most tissues increased substantially. The progress rate of WSSV in different tissues during the infection was different and the most favorite replication tissue of WSSV in early infection is the stomach, followed by the gill. The heaviest infected tissues are the skin, stomach and gill, explaining the symptoms of skin color changes, reduction of food consumption and gathering at the water surface due to dyspnea. The hepatopancreas, muscle and nerve were not the main replication sites in advanced infection. Also, the expression profiles of two anti-virus genes FcALF and FcAV were analyzed. This study might provide important data to study the virus infection mechanism. (C) 2012 Elsevier B.V. All rights reserved.
英文摘要	White spot syndrome virus (WSSV) is the most severe viral pathogen to the shrimp culture industry worldwide. The temporal and spatial dynamic changes of WSSV in Chinese shrimp Fenneropenaeus chinensis maintained at 22 +/- 1 degrees C during infection via injection were studied through the quantitative real-time PCR method. The pleopods were selected for virus quantification at 0, 6, 12, 18, 24, 27, 30, 33, 36, 39, 42, 45 and 48 hours post-injection (hpi). Besides the pleopod, different tissues including the hepatopancreas, nerve, heart, eyestalk, muscle, lymphoid organ, gut, stomach, gill and skin were also selected for viral load analysis at 0, 12, 27 and 30 hpi as well as the moribund stage after 40 hpi. The curve of viral load change in vivo assumed a very similar shape with the typical viral one-step growth curve. The infection process was classified into three stages: the light infection stage (0 to 18 hpi) when the viral load in the pleopod was at the level of nomore than 10(3)/ng DNA and no mortality appeared; the moderate infection stage (18 to 45 hpi), corresponding to the logarithmic phase of WSSV replication, when the viral load in the pleopod ranged from 10(3) to 10(5)/ng DNA and a low mortality rate was observed; and the heavy infection stage when WSSV propagation went into the plateau phage and massive mortality happened. A turning point from chronic infection to acute infection was suggested from 27 to 30 hpi when the viral load in most tissues increased substantially. The progress rate of WSSV in different tissues during the infection was different and the most favorite replication tissue of WSSV in early infection is the stomach, followed by the gill. The heaviest infected tissues are the skin, stomach and gill, explaining the symptoms of skin color changes, reduction of food consumption and gathering at the water surface due to dyspnea. The hepatopancreas, muscle and nerve were not the main replication sites in advanced infection. Also, the expression profiles of two anti-virus genes FcALF and FcAV were analyzed. This study might provide important data to study the virus infection mechanism. (C) 2012 Elsevier B.V. All rights reserved.
学科主题	Fisheries ; Marine & Freshwater Biology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Marine & Freshwater Biology
研究领域[WOS]	Fisheries ; Marine & Freshwater Biology
关键词[WOS]	WHITE-SPOT-SYNDROME ; SYNDROME VIRUS WSSV ; POLYMERASE-CHAIN-REACTION ; REAL-TIME PCR ; PENAEUS-MONODON ; ANTILIPOPOLYSACCHARIDE FACTOR ; BACULOVIRUS WSBV ; QUANTITATIVE PCR ; GENOME SEQUENCE ; GENE
收录类别	SCI
原文出处	10.1016/j.aquaculture.2012.11.014
语种	英语
WOS记录号	WOS:000313569100018
公开日期	2014-07-17
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/16526]
专题	海洋研究所_实验海洋生物学重点实验室
作者单位	Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Sun, Yumiao,Li, Fuhua,Xiang, Jianhai. Analysis on the dynamic changes of the amount of WSSV in Chinese shrimp Fenneropenaeus chinensis during infection[J]. AQUACULTURE,2013,376:124-132.
APA	Sun, Yumiao,Li, Fuhua,&Xiang, Jianhai.(2013).Analysis on the dynamic changes of the amount of WSSV in Chinese shrimp Fenneropenaeus chinensis during infection.AQUACULTURE,376,124-132.
MLA	Sun, Yumiao,et al."Analysis on the dynamic changes of the amount of WSSV in Chinese shrimp Fenneropenaeus chinensis during infection".AQUACULTURE 376(2013):124-132.