A Potentiometric Flow Biosensor Based on Ammonia-Oxidizing Bacteria for the Detection of Toxicity in Water

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	A Potentiometric Flow Biosensor Based on Ammonia-Oxidizing Bacteria for the Detection of Toxicity in Water
	Zhang, Qianyu 1,2; Ding, Jiawang1 ; Kou, Lijuan 1,2; Qin, Wei1
刊名	SENSORS
	2013-06-01
卷号	13 期号:6 页码:6936-6945
关键词	potentiometry flow biosensors ion-selective electrodes toxicity Nitrosomonas europaea
ISSN号	1424-8220
通讯作者	Qin, W (reprint author), Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC,YICCAS, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China. qyzhang@yic.ac.cn ; jwding@yic.ac.cn ; ljkou@yic.ac.cn ; wqin@yic.ac.cn
产权排序	[Zhang, Qianyu; Ding, Jiawang; Kou, Lijuan; Qin, Wei] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC,YICCAS, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China; [Zhang, Qianyu; Kou, Lijuan] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
中文摘要	A flow biosensor for the detection of toxicity in water using the ammonia-oxidizing bacterium (AOB) Nitrosomonas europaea as a bioreceptor and a polymeric membrane ammonium-selective electrode as a transducer is described. The system is based on the inhibition effects of toxicants on the activity of AOB, which can be evaluated by measuring the ammonium consumption rates with the ammonium-selective membrane electrode. The AOB cells are immobilized on polyethersulfone membranes packed in a holder, while the membrane electrode is placed downstream in the flow cell. Two specific inhibitors of the ammonia oxidation. allylthiourea and thioacetamide. have been tested. The IC50 values defined as the concentration of an inhibitor causing a 50% reduction in the ammonia oxidation activity have been measured as 0.17 mu M and 0.46 mu M for allylthiourea and thioacetamide, respectively. The proposed sensor offers advantages of simplicity, speed and high sensitivity for measuring toxicity in water.
英文摘要	A flow biosensor for the detection of toxicity in water using the ammonia-oxidizing bacterium (AOB) Nitrosomonas europaea as a bioreceptor and a polymeric membrane ammonium-selective electrode as a transducer is described. The system is based on the inhibition effects of toxicants on the activity of AOB, which can be evaluated by measuring the ammonium consumption rates with the ammonium-selective membrane electrode. The AOB cells are immobilized on polyethersulfone membranes packed in a holder, while the membrane electrode is placed downstream in the flow cell. Two specific inhibitors of the ammonia oxidation. allylthiourea and thioacetamide. have been tested. The IC50 values defined as the concentration of an inhibitor causing a 50% reduction in the ammonia oxidation activity have been measured as 0.17 mu M and 0.46 mu M for allylthiourea and thioacetamide, respectively. The proposed sensor offers advantages of simplicity, speed and high sensitivity for measuring toxicity in water.
学科主题	Chemistry, Analytical ; Electrochemistry ; Instruments & Instrumentation
研究领域[WOS]	Chemistry ; Electrochemistry ; Instruments & Instrumentation
关键词[WOS]	NITROSOMONAS-EUROPAEA ; NITRIFICATION ; INHIBITORS ; BIOASSAY
收录类别	SCI
资助信息	Instrument Developing Project of the Chinese Academy of Sciences [YZ201161]; National Natural Science Foundation of China [41176081, 21207156]; Taishan Scholar Program of Shandong Province [TS20081159]
原文出处	http://dx.doi.org/10.3390/s130606936
语种	英语
WOS记录号	WOS:000320767600004
公开日期	2014-07-08
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/6986]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC,YICCAS, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Shandong Prov Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Qianyu,Ding, Jiawang,Kou, Lijuan,et al. A Potentiometric Flow Biosensor Based on Ammonia-Oxidizing Bacteria for the Detection of Toxicity in Water[J]. SENSORS,2013,13(6):6936-6945.
APA	Zhang, Qianyu,Ding, Jiawang,Kou, Lijuan,&Qin, Wei.(2013).A Potentiometric Flow Biosensor Based on Ammonia-Oxidizing Bacteria for the Detection of Toxicity in Water.SENSORS,13(6),6936-6945.
MLA	Zhang, Qianyu,et al."A Potentiometric Flow Biosensor Based on Ammonia-Oxidizing Bacteria for the Detection of Toxicity in Water".SENSORS 13.6(2013):6936-6945.