Single-nucleotide polymorphism genotyping using a novel multiplexed electrochemical biosensor with nonfouling surface

CORC > 上海应用物理研究所 > 中国科学院上海应用物理研究所 > 中科院上海应用物理研究所2011-2017年

	Single-nucleotide polymorphism genotyping using a novel multiplexed electrochemical biosensor with nonfouling surface
	Liu, Gang ; Lao, Ruojun ; Xu, Li ; Xu, Qin ; Li, Lanying ; Zhang, Min ; Song, Shiping ; Fan, Chunhai
刊名	BIOSENSORS & BIOELECTRONICS
	2013
卷号	42 页码:CONCATENATE(Sheet1!I303,-Sheet1!J303)
ISSN号	0956-5663
英文摘要	A novel electrochemical DNA biosensor for single-nucleotide polymorphism (SNP) analysis was developed. In this work, an oligonucleotide-incorporated nonfouling surface (ONS) was constructed to resist nonspecific absorption. The biosensor was developed using a 16-electrode array for high-throughput SNP analysis. The proposed strategy was primarily based on specific oligonucleotide ligation. Fully matched target DNA templated the ligation between a capture probe assembled on gold electrodes and a tandem signal probe with a biotin moiety that could capture avidin-horseradish peroxidase and sequentially generate a catalysed amperometric signal. A pre-core mutation in the hepatitis B virus (HBV) genome at G1896A and two adjacent polymorphisms in the human CYP2C19 genome at C680T and G681A were analysed. Polymerase chain reaction (PCR) products were used as real-life samples and analysed. Our results showed that 10% of a single-mismatched mutant gene was clearly distinguished with a current signal 16 times higher than that of the blank sample, demonstrating the selectivity and practicability of the multiplexed electrochemical DNA biosensor. (C) 2012 Elsevier B.V. All rights reserved.
收录类别	SCI
语种	英语
WOS记录号	WOS:000319951700085
公开日期	2014-06-13
内容类型	期刊论文
源URL	[http://ir.sinap.ac.cn/handle/331007/13903]
专题	上海应用物理研究所_中科院上海应用物理研究所2011-2017年
推荐引用方式 GB/T 7714	Liu, Gang,Lao, Ruojun,Xu, Li,et al. Single-nucleotide polymorphism genotyping using a novel multiplexed electrochemical biosensor with nonfouling surface[J]. BIOSENSORS & BIOELECTRONICS,2013,42:CONCATENATE(Sheet1!I303,-Sheet1!J303).
APA	Liu, Gang.,Lao, Ruojun.,Xu, Li.,Xu, Qin.,Li, Lanying.,...&Fan, Chunhai.(2013).Single-nucleotide polymorphism genotyping using a novel multiplexed electrochemical biosensor with nonfouling surface.BIOSENSORS & BIOELECTRONICS,42,CONCATENATE(Sheet1!I303,-Sheet1!J303).
MLA	Liu, Gang,et al."Single-nucleotide polymorphism genotyping using a novel multiplexed electrochemical biosensor with nonfouling surface".BIOSENSORS & BIOELECTRONICS 42(2013):CONCATENATE(Sheet1!I303,-Sheet1!J303).