In vitro biotransformation of PBDEs by root crude enzyme extracts: Potential role of nitrate reductase (NaR) and glutathione S-transferase (GST) in their debromination

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	In vitro biotransformation of PBDEs by root crude enzyme extracts: Potential role of nitrate reductase (NaR) and glutathione S-transferase (GST) in their debromination
	Huang, Honglin ; Zhang, Shuzhen ; Wang, Sen ; Lv, Jitao
刊名	CHEMOSPHERE
	2013
卷号	90 期号:6 页码:1885-1892
通讯作者	Zhang, SZ
中文摘要	In order to investigate the enzyme transformation of PBDEs and to track the key enzymes involved in PBDE degradation in plants, in vivo exposure of plants of ryegrass, pumpkin and maize and in vitro exposure of their root crude enzyme extracts to PBDEs were conducted. Degradation of PBDEs in the root crude enzyme solutions fit well with the first order kinetics (R-2 = 0.52-0.97, P < 0.05), and higher PBDEs degraded faster than the lower ones. PBDEs could be transformed to lower brominated PBDEs and hydroxylated-PBDEs by the root crude enzyme extracts with debromination as the main pathway which contributed over 90% of PBDE depletion. In vitro and in vivo exposure to PBDEs produced similar responses in root enzyme activities of which the nitroreductase (NaR) and glutathione-transferase (GST) activities decreased significantly, while the peroxidase, catalase and cytochrome P-450 activities had no significant changes. Furthermore, higher enzyme concentrations of NaR and GST led to higher PBDE debromination rates, and the time-dependent activities of NaR and GST in the root crude enzyme extracts were similar to the trends of PBDE depletion. All these results suggest that NaR and GST were the key enzymes responsible for PBDE degradation. This conclusion was further confirmed by the in vitro debromination of PBDEs with the commercial pure NaR and GST. (C) 2012 Elsevier Ltd. All rights reserved.
收录类别	SCI
WOS记录号	WOS:000315133400017
公开日期	2014-06-06
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/7534]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Huang, Honglin,Zhang, Shuzhen,Wang, Sen,et al. In vitro biotransformation of PBDEs by root crude enzyme extracts: Potential role of nitrate reductase (NaR) and glutathione S-transferase (GST) in their debromination[J]. CHEMOSPHERE,2013,90(6):1885-1892.
APA	Huang, Honglin,Zhang, Shuzhen,Wang, Sen,&Lv, Jitao.(2013).In vitro biotransformation of PBDEs by root crude enzyme extracts: Potential role of nitrate reductase (NaR) and glutathione S-transferase (GST) in their debromination.CHEMOSPHERE,90(6),1885-1892.
MLA	Huang, Honglin,et al."In vitro biotransformation of PBDEs by root crude enzyme extracts: Potential role of nitrate reductase (NaR) and glutathione S-transferase (GST) in their debromination".CHEMOSPHERE 90.6(2013):1885-1892.