Hydrogen peroxide/ATR-Chk2 activation mediates p53 protein stabilization and anti-cancer activity of cheliensisin A in human cancer cells

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 植物化学与西部植物资源持续利用国家重点实验室

	Hydrogen peroxide/ATR-Chk2 activation mediates p53 protein stabilization and anti-cancer activity of cheliensisin A in human cancer cells
	Zhang, Jingjie 1; Gao, Guangxun 1,2; Chen, Liang 1; Li, Jingxia 1; Deng, Xu3 ; Zhao, Qin-shi3 ; Huang, Chuanshu 1
刊名	ONCOTARGET
	2014
卷号	5 期号:3 页码:841-852
关键词	Cheliensisin A Apoptosis colon cancer p53 chemotherapeutic agent
ISSN号	1949-2553
通讯作者	Huang, CS (reprint author), NYU, Sch Med, Nelson Inst Environm Med, Tuxedo Pk, NY 10987 USA. ; qinshizhao@mail.kib.ac.cn ; chuanshu.huang@nyumc.org
英文摘要	Cheliensisine A (Chel A) as a novel styryl-lactone isolated from Goniothalamus cheliensis Hu has been indicated to be a chemotherapeutic agent in Leukemia HL-60 cells. However, its potential for cancer treatment and the underlying mechanisms are not deeply investigated to the best of our knowledge. Current studies showed that Chel A could trigger p53-mediated apoptosis, accompanied with dramatically inhibition of anchorage-independent growth of human colon cancer HCT116 cells. Further studies found that Chel A treatment resulted in p53 protein stabilization and accumulation via the induction of its phosphorylation at Ser20 and Ser15. Moreover, Chel A-induced p53 protein accumulation and activation required ATR/Chk2 axis, which is distinct from the mechanism that we have most recently identified the Chk1/p53-dependent apoptotic response by Chel A in normal mouse epidermal Cl41 cells. In addition, our results demonstrated that hydrogen peroxide generation induced by Chel A acted as a precursor for all these signaling events and downstream biological effects. Taken together, we have identified the Chel A as a new therapeutic agent, which highlights its potential for cancer therapeutic effect.
学科主题	Oncology ; Cell Biology
类目[WOS]	Oncology ; Cell Biology
研究领域[WOS]	Oncology ; Cell Biology
关键词[WOS]	DNA-DAMAGE ; INDUCED PHOSPHORYLATION ; DOWN-REGULATION ; APOPTOSIS ; EXPRESSION ; INDUCTION ; PATHWAY ; TRANSFORMATION ; HYPEROXIA ; MDM2
收录类别	SCI
资助信息	NIH/NCI [RO1 CA177665, CA165980, RO1 CA112557]; NIH/NIEHS [ES000260]; [NSFC81229002]; [NSF90813004]
语种	英语
WOS记录号	WOS:000332701300023
公开日期	2014-05-16
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/18052]
专题	昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
作者单位	1.NYU, Sch Med, Nelson Inst Environm Med, Tuxedo Pk, NY 10987 USA 2.Fourth Mil Med Univ, Xijing Hosp, Dept Hematol, Xian 710032, Shaanxi, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Jingjie,Gao, Guangxun,Chen, Liang,et al. Hydrogen peroxide/ATR-Chk2 activation mediates p53 protein stabilization and anti-cancer activity of cheliensisin A in human cancer cells[J]. ONCOTARGET,2014,5(3):841-852.
APA	Zhang, Jingjie.,Gao, Guangxun.,Chen, Liang.,Li, Jingxia.,Deng, Xu.,...&Huang, Chuanshu.(2014).Hydrogen peroxide/ATR-Chk2 activation mediates p53 protein stabilization and anti-cancer activity of cheliensisin A in human cancer cells.ONCOTARGET,5(3),841-852.
MLA	Zhang, Jingjie,et al."Hydrogen peroxide/ATR-Chk2 activation mediates p53 protein stabilization and anti-cancer activity of cheliensisin A in human cancer cells".ONCOTARGET 5.3(2014):841-852.