The neck linker of kinesin 1 seems optimally designed to approach the largest stepping velocity: a simulation study of an ideal model

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	The neck linker of kinesin 1 seems optimally designed to approach the largest stepping velocity: a simulation study of an ideal model
	Li, M; Ou-Yang, ZC
刊名	JOURNAL OF PHYSICS-CONDENSED MATTER
	2012
卷号	24 期号:3 页码:35105
关键词	HAND-OVER-HAND MICROTUBULE-BASED MOTILITY ATP HYDROLYSIS MOTOR DOMAINS FORCE-FIELD INTERNAL STRAIN SWING MODEL PROCESSIVITY MECHANISM STEPS
ISSN号	0953-8984
通讯作者	Shu, YG (reprint author), Chinese Acad Sci, Inst Theoret Phys, Beijing 100190, Peoples R China.
英文摘要	The neck linker is widely believed to play a critical role in the hand-over-hand walking of conventional kinesin 1. Experiments have shown that change of the neck linker length will significantly change the stepping velocity of the motor. In this paper, we studied this length effect based on a highly simplified chemically powered ratchet model. In this model, we assume that the chemical steps (ATP hydrolysis, ADP and P(i) release, ATP binding, neck linker docking) are fast enough under conditions far from equilibrium and the mechanical steps (detachment, diffusional search and re-attachment of the free head) are rate-limiting in kinesin walking. According to this model, and regarding the neck linker as a worm-like-chain polypeptide, we can calculate the steady state stepping velocity of the motor for different neck linker lengths. Our results show, under the actual values of binding energy between kinesin head and microtubule (similar to 15k(BT)) and the persistence length of neck linker (similar to 0.5 nm), that there is an optimal neck linker length (similar to 14-16 a.a.) corresponding to the maximal velocity, which implies that the length of the wild-type neck linker (similar to 15 a.a.) might be optimally designed for kinesin 1 to approach the largest stepping velocity.
学科主题	Physics
收录类别	SCI
资助信息	National Natural Science Foundation of China [91027045, 11075015]; National Basic Research Program of China (973 Program) [2007CB935903]
原文出处	http://dx.doi.org/10.1088/0953-8984/24/3/035105
语种	英语
WOS记录号	WOS:000298641600005
公开日期	2014-04-25
内容类型	期刊论文
源URL	[http://ir.itp.ac.cn/handle/311006/15178]
专题	理论物理研究所_理论物理所1978-2010年知识产出
推荐引用方式 GB/T 7714	Li, M,Ou-Yang, ZC. The neck linker of kinesin 1 seems optimally designed to approach the largest stepping velocity: a simulation study of an ideal model[J]. JOURNAL OF PHYSICS-CONDENSED MATTER,2012,24(3):35105.
APA	Li, M,&Ou-Yang, ZC.(2012).The neck linker of kinesin 1 seems optimally designed to approach the largest stepping velocity: a simulation study of an ideal model.JOURNAL OF PHYSICS-CONDENSED MATTER,24(3),35105.
MLA	Li, M,et al."The neck linker of kinesin 1 seems optimally designed to approach the largest stepping velocity: a simulation study of an ideal model".JOURNAL OF PHYSICS-CONDENSED MATTER 24.3(2012):35105.