Phosphoprotein/Phosphopeptide Enrichment and Analysis Based on Nanostructured Materials

	Phosphoprotein/Phosphopeptide Enrichment and Analysis Based on Nanostructured Materials
	Cheng G ; Wang ZG ; Liu YL ; Zhang JL ; Sun DH ; Ni JZ
刊名	progress in chemistry
	2013
卷号	25 期号:4 页码:620-632
关键词	CORE-SHELL MICROSPHERES MALDI-TOF-MS IONIZATION MASS-SPECTROMETRY TIO2 NANOCRYSTAL CLUSTERS HIGHLY SPECIFIC CAPTURE SELECTIVE ENRICHMENT PHOSPHORYLATED PEPTIDES PHOSPHOPEPTIDE ENRICHMENT PHOSPHOPROTEOME ANALYSIS PROTEIN-PHOSPHORYLATION
ISSN号	1005-281x
通讯作者	zhang jl
中文摘要	protein phosphorylation is one of the most ubiquitous post-translational modifications. many low-abundance endogenous phosphoproteins and phosphopeptides in the body fluids or tissues are biomarkers with higher clinical sensitivity and specificity, which could provide valuable information for the detection of many diseases and elucidation of pathology. it is still a great challenge to detect the phosphoproteins and phosphopeptides from complex biological samples directly due to reversibility of protein phosphorylation and the extremely low concentrations of phosphoproteins. nanostructured materials have attracted particular attentions in enrichment, separation, and purification of phosphoproteins/phosphopeptides due to their larger surface area, numerous affinity sites and unique structures. the research subject has become one of research hotspots in phosphoproteomics presently. various multifunctional nanostructured materials such as core-shell particles with mesoporous affinity shell and magnetic core, hybrids of multiple components and composite affinity materials have been synthesized for selective enrichment and fast purification of phosphoproteins/phosphopeptides. in this review, we are focusing on recent advancements of nanostructured materials for phosphoprotein/phosphopeptide enrichment and purification prior to ms analysis. definition, structure characteristic, unique physicochemical properties and potential in bioseparation of the nanostructured materials are first introduced. subsequently, the related research background on phosphoproteomic studies in proteomics using mass spectrometric strategies in combination with phosphospecific enrichment techniques is briefly presented. after that, two types of affinity enrichment mechanisms to phosphoproteins/phosphopeptides are compactly discussed. next, mesoporous, hybrid, and composite nanostructured materials for enrichment of phosphoproteins/phosphopeptides as well as application of multifunctional nanostructured materials in phosphoproteomics are summarized in detail. finally, some unsolved problems and a brief perspective and outlook on phosphopeptide enrichment are proposed.
收录类别	SCI收录期刊论文
语种	中文
WOS记录号	WOS:000318131600019
公开日期	2014-04-20
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/50484]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Cheng G,Wang ZG,Liu YL,et al. Phosphoprotein/Phosphopeptide Enrichment and Analysis Based on Nanostructured Materials[J]. progress in chemistry,2013,25(4):620-632.
APA	Cheng G,Wang ZG,Liu YL,Zhang JL,Sun DH,&Ni JZ.(2013).Phosphoprotein/Phosphopeptide Enrichment and Analysis Based on Nanostructured Materials.progress in chemistry,25(4),620-632.
MLA	Cheng G,et al."Phosphoprotein/Phosphopeptide Enrichment and Analysis Based on Nanostructured Materials".progress in chemistry 25.4(2013):620-632.