Purification, structural elucidation and antitumor activity of a novel mannogalactoglucan from the fruiting bodies of Lentinus edodes | |
Jeff IB ; Li SS ; Peng XX ; Kassim RMR ; Liu BF ; Zhou YF | |
刊名 | fitoterapia |
2013 | |
卷号 | 84页码:338-346 |
关键词 | POLYSACCHARIDES MUSHROOMS |
ISSN号 | 0367-326x |
通讯作者 | liu bf |
中文摘要 | a mannogalactoglucan, named le-mgg, was isolated from the basidiocarps of lentinus edodes by hot water-extraction, ethanol precipitation anion exchange chromatography, and further purified by gel-permeation chromatography (gpc). its structural features were investigated by high performance liquid chromatography (hplc), high performance gel-permeation chromatography (hpgpc), methylation analysis, periodate oxidation-smith degradation, and by ir and nmr spectroscopy, including two-dimensional (2d) nmr. hplc analysis revealed that le-mgg contained mannose-galactose-glucose in the molar ratio of 10:18:72. gpc and hpgpc showed that le-mgg was a homogeneous fraction (d = 1.34) and its molecular weight was estimated to be 18 kda. chemical and spectroscopic studies indicated that le-mgg consists of (1 -> 6)-, (1 -> 4)- and (1 -> 3)-linked beta-d-glucopyranosyl residues, (1 -> 6)-linked alpha-d-galactopyranosyl residues, (1 -> 3,6)- and (1 -> 2,4)-linked alpha-d-mannopyranosyl residues and terminal residues of beta-d-glucopyranosyl. cytotoxicity assay showed that le-mgg presented higher antitumor activities against s-180 cell with a dose-dependent manner, and exhibited lower cytotoxicity to carcinoma hct-116 and ht-29 cells. our studies showed also that le-mgg presented antitumor bioactivities on sarcoma 180 solid tumor cell implanted in kunming mice. this finding suggests that mannogalactoglucan should be explored as potential antitumor agents and could be potentially applied as a natural antitumor drug. (c) 2012 elsevier b.v. all rights reserved. |
收录类别 | SCI收录期刊论文 |
语种 | 英语 |
WOS记录号 | WOS:000316531400048 |
公开日期 | 2014-04-18 |
内容类型 | 期刊论文 |
源URL | [http://ir.ciac.jl.cn/handle/322003/50322] |
专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
推荐引用方式 GB/T 7714 | Jeff IB,Li SS,Peng XX,et al. Purification, structural elucidation and antitumor activity of a novel mannogalactoglucan from the fruiting bodies of Lentinus edodes[J]. fitoterapia,2013,84:338-346. |
APA | Jeff IB,Li SS,Peng XX,Kassim RMR,Liu BF,&Zhou YF.(2013).Purification, structural elucidation and antitumor activity of a novel mannogalactoglucan from the fruiting bodies of Lentinus edodes.fitoterapia,84,338-346. |
MLA | Jeff IB,et al."Purification, structural elucidation and antitumor activity of a novel mannogalactoglucan from the fruiting bodies of Lentinus edodes".fitoterapia 84(2013):338-346. |
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