The Asymmetrical Structure of Golgi Apparatus Membranes Revealed by In situ Atomic Force Microscope

	The Asymmetrical Structure of Golgi Apparatus Membranes Revealed by In situ Atomic Force Microscope
	Xu HJ ; Su WH ; Cai MJ ; Jiang JG ; Zeng XL ; Wang HD
刊名	plos one
	2013
卷号	8 期号:4 页码:文献号: e61596
关键词	LIPID RAFTS ELECTRON-MICROSCOPY CELL-MEMBRANES BIOLOGICAL-MEMBRANES MODEL MEMBRANES CHOLESTEROL COMPLEX TRANSPORT SURFACE DOMAINS
ISSN号	1932-6203
通讯作者	zeng xl
中文摘要	the golgi apparatus has attracted intense attentions due to its fascinating morphology and vital role as the pivot of cellular secretory pathway since its discovery. however, its complex structure at the molecular level remains elusive due to limited approaches. in this study, the structure of golgi apparatus, including the golgi stack, cisternal structure, relevant tubules and vesicles, were directly visualized by high-resolution atomic force microscope. we imaged both sides of golgi apparatus membranes and revealed that the outer leaflet of golgi membranes is relatively smooth while the inner membrane leaflet is rough and covered by dense proteins. with the treatment of methyl-b-cyclodextrin and triton x-100, we confirmed the existence of lipid rafts in golgi apparatus membrane, which are mostly in the size of 20 nm -200 nm and appear irregular in shape. our results may be of significance to reveal the structure-function relationship of the golgi complex and pave the way for visualizing the endomembrane system in mammalian cells at the molecular level.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000317893400076
公开日期	2014-04-16
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/49906]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Xu HJ,Su WH,Cai MJ,et al. The Asymmetrical Structure of Golgi Apparatus Membranes Revealed by In situ Atomic Force Microscope[J]. plos one,2013,8(4):文献号: e61596.
APA	Xu HJ,Su WH,Cai MJ,Jiang JG,Zeng XL,&Wang HD.(2013).The Asymmetrical Structure of Golgi Apparatus Membranes Revealed by In situ Atomic Force Microscope.plos one,8(4),文献号: e61596.
MLA	Xu HJ,et al."The Asymmetrical Structure of Golgi Apparatus Membranes Revealed by In situ Atomic Force Microscope".plos one 8.4(2013):文献号: e61596.