Microbial surface display of glucose dehydrogenase for amperometric glucose biosensor

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 生物传感技术团队

	Microbial surface display of glucose dehydrogenase for amperometric glucose biosensor
	Liang, Bo 1,2; Li, Liang 1,2,3; Tang, Xiangliang 1,2; Lang, Qiaolin 1,2; Wang, Hongwei 1,2; Li, Feng 3; Shi, Jianguo 4; Shen, Wei 5; Palchetti, Ilaria 6; Mascini, Marco 6
刊名	BIOSENSORS & BIOELECTRONICS
	2013-07-15
卷号	45 期号:2013 页码:19-24
关键词	Glucose dehydrogenase displayed bacteria Bacterial surface display Electrochemical glucose biosensor Bacterial electrode Saccharide analysis
英文摘要	A genetically engineered Escherichia coil (E. coil) strain displaying glucose dehydrogenase (GDH) with ice-nucleation protein (INP) as the anchoring motif was first constructed. The surface localization and functionality of the fusion protein containing GDH were verified by SDS-PAGE, Western blotting and enzymatic activity assay. The fusion of INP had no effects on the functionality of GDH cofactor binding domain. The activity assay showed that 74.6% of the cell lysate GDH activity was detected in the outer membrane fractions. Compared with the crude enzyme solution from E. coli expressing intracellular GDH, the GDH-displaying bacteria (GDH-bacteria) was stable within pH 6-10 below 40 degrees C. Further, a novel electrochemical glucose biosensor was developed by construction of Nafion/GDH-bacteria/multiwalled-carbon-nanotube modified electrode. The as-prepared biosensor is linear with the concentration of D-glucose within the range of 50-800 mu M and a low detection limit of 4 mu M D-glucose (S/N=3). Excess saccharides including D-galactose, D-fructose, D-cellbiose, L-arabinose and D-sucrose, D-maltose, D-mannose and D-xylose as well as common interfering substances (acetaminophen, ascorbic acid and uric acid) did not affect the detection of D-glucose (0.1 mM). The proposed biosensor is stable, specific, reproducible, simple, rapid and cost-effective, which can be used for detection of real samples. It is envisioned that this GDH-bacteria will be found promising applications in biofuel cell, glucose detection and cofactor reproduction system. (C) 2013 Elsevier B.V. All rights reserved.
学科主题	生物传感器技术
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry, Analytical ; Electrochemistry ; Nanoscience & Nanotechnology
研究领域[WOS]	Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
关键词[WOS]	ICE NUCLEATION PROTEIN ; N-TERMINAL DOMAIN ; ELECTRON-TRANSFER ; ESCHERICHIA-COLI ; ORGANOPHOSPHORUS HYDROLASE ; BACILLUS-SUBTILIS ; OXIDASE ; IMMOBILIZATION ; ENZYMES ; SYSTEM
收录类别	SCI
语种	英语
WOS记录号	WOS:000317888600004
公开日期	2014-03-24
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn:8080/handle/337004/1688]
专题	青岛生物能源与过程研究所_生物传感技术团队
作者单位	1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Lab Biosensing, Qingdao 266101, Peoples R China 2.Chinese Acad Sci, Key Lab Bioenergy, Qingdao 266101, Peoples R China 3.Qingdao Univ Sci & Technol, Coll Environm & Safety Engn, Qingdao 266042, Peoples R China 4.Shandong Acad Sci, Inst Biol, Key Lab Biosensors Shangdong Prov, Jinan 250014, Peoples R China 5.Monash Univ, Dept Chem Engn, Clayton, Vic 3800, Australia 6.Univ Florence, Dipartimento Chim, I-50019 Sesto Fiorentino, Italy
推荐引用方式 GB/T 7714	Liang, Bo,Li, Liang,Tang, Xiangliang,et al. Microbial surface display of glucose dehydrogenase for amperometric glucose biosensor[J]. BIOSENSORS & BIOELECTRONICS,2013,45(2013):19-24.
APA	Liang, Bo.,Li, Liang.,Tang, Xiangliang.,Lang, Qiaolin.,Wang, Hongwei.,...&Liu, Aihua.(2013).Microbial surface display of glucose dehydrogenase for amperometric glucose biosensor.BIOSENSORS & BIOELECTRONICS,45(2013),19-24.
MLA	Liang, Bo,et al."Microbial surface display of glucose dehydrogenase for amperometric glucose biosensor".BIOSENSORS & BIOELECTRONICS 45.2013(2013):19-24.