Isolation and identification of a lethal rhabdovirus from farmed rice field eels Monopterus albus

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	Isolation and identification of a lethal rhabdovirus from farmed rice field eels Monopterus albus
	Ou, Tong; Zhu, Ruo-Lin; Chen, Zhong-Yuan; Zhang, Qi-Ya
刊名	DISEASES OF AQUATIC ORGANISMS
	2013-11-06
卷号	106 期号:3 页码:197-206
关键词	Fish viral disease Rice field eel Monopterus albus rhabdovirus MoARV Histopathology Electron microscopy Phylogenetic analysis
ISSN号	0177-5103
通讯作者	Zhang, QY (reprint author), Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
中文摘要	We provide the first description of a virus responsible for a systemic hemorrhagic disease causing high mortality in farmed rice field eels Monopterus albus in China. Typical signs exhibited by the diseased fish were extensive hemorrhages in the skin and viscera and some neuro logical signs, such as loss of equilibrium and disorganized swimming. Histopathological examination revealed various degrees of necrosis within the spleen and liver. Virus isolation was attempted from visceral tissues of diseased fish by inoculation on 6 fish cell lines. Typical cytopathic effects (CPE) were produced in bluegill fry (BF2) cells, so this cell line was chosen for further isolation and propagation of the virus. Electron microscopy observation showed that the negative stained viral particles had the characteristic bullet shape of rhabdoviruses and an estimated size of 60 x 120 nm. We therefore tentatively refer to this virus as Monopterus albus rhabdovirus (MoARV). Molecular characterization of MoARV, including sequence analysis of the nucleoprotein (N), phosphoprotein (P), and glycoprotein (G) genes, revealed 94.5 to 97.3% amino acid similarity to that of Siniperca chuatsi rhabdovirus. Phylogenetic analysis based on the amino acid sequences of N and G proteins indicated that MoARV should be a member of the genus Vesiculovirus. Koch's postulates were fulfilled by infecting healthy rice field eels with MoARV, which produced an acute infection. RT-PCR analysis demonstrated that MoARV RNA could be detected in both naturally and experimentally infected fish. The data suggest that MoARV was the causative pathogen of the disease.
英文摘要	We provide the first description of a virus responsible for a systemic hemorrhagic disease causing high mortality in farmed rice field eels Monopterus albus in China. Typical signs exhibited by the diseased fish were extensive hemorrhages in the skin and viscera and some neuro logical signs, such as loss of equilibrium and disorganized swimming. Histopathological examination revealed various degrees of necrosis within the spleen and liver. Virus isolation was attempted from visceral tissues of diseased fish by inoculation on 6 fish cell lines. Typical cytopathic effects (CPE) were produced in bluegill fry (BF2) cells, so this cell line was chosen for further isolation and propagation of the virus. Electron microscopy observation showed that the negative stained viral particles had the characteristic bullet shape of rhabdoviruses and an estimated size of 60 x 120 nm. We therefore tentatively refer to this virus as Monopterus albus rhabdovirus (MoARV). Molecular characterization of MoARV, including sequence analysis of the nucleoprotein (N), phosphoprotein (P), and glycoprotein (G) genes, revealed 94.5 to 97.3% amino acid similarity to that of Siniperca chuatsi rhabdovirus. Phylogenetic analysis based on the amino acid sequences of N and G proteins indicated that MoARV should be a member of the genus Vesiculovirus. Koch's postulates were fulfilled by infecting healthy rice field eels with MoARV, which produced an acute infection. RT-PCR analysis demonstrated that MoARV RNA could be detected in both naturally and experimentally infected fish. The data suggest that MoARV was the causative pathogen of the disease.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries ; Veterinary Sciences
研究领域[WOS]	Fisheries ; Veterinary Sciences
关键词[WOS]	HEMORRHAGIC SEPTICEMIA VIRUS ; MANDARIN FISH ; GENOMIC SEQUENCE ; SPRING VIREMIA ; CARP VIRUS ; EUROPEAN X ; MORTALITY ; POSITION ; PERCH ; GENE
收录类别	SCI
资助信息	National Major Basic Research Program [2010CB-126303]; National Natural Science Foundation of China [31072239, 31270213]; FEBL research grant [2011-FBZ12, 2008FBZ16]
语种	英语
WOS记录号	WOS:000326653800002
公开日期	2014-01-24
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/19769]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Ou, Tong,Zhu, Ruo-Lin,Chen, Zhong-Yuan,et al. Isolation and identification of a lethal rhabdovirus from farmed rice field eels Monopterus albus[J]. DISEASES OF AQUATIC ORGANISMS,2013,106(3):197-206.
APA	Ou, Tong,Zhu, Ruo-Lin,Chen, Zhong-Yuan,&Zhang, Qi-Ya.(2013).Isolation and identification of a lethal rhabdovirus from farmed rice field eels Monopterus albus.DISEASES OF AQUATIC ORGANISMS,106(3),197-206.
MLA	Ou, Tong,et al."Isolation and identification of a lethal rhabdovirus from farmed rice field eels Monopterus albus".DISEASES OF AQUATIC ORGANISMS 106.3(2013):197-206.