Structure of the formate transporter FocA reveals a pentameric aquaporin-like channel

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	Structure of the formate transporter FocA reveals a pentameric aquaporin-like channel
	Wang, Y ; Huang, YJ ; Wang, JW ; Cheng, C ; Huang, WJ ; Lu, PL ; Xu, YN ; Wang, PY ; Yan, N ; Shi, YG
刊名	NATURE
	2009
卷号	462 期号:7272 页码:467
关键词	ESCHERICHIA-COLI WATER CHANNEL MEMBRANE-PROTEINS NITRATE SELECTIVITY SOFTWARE GLYCEROL NARU IDENTIFICATION RECONSTITUTION
ISSN号	0028-0836
通讯作者	Yan, N (reprint author), Tsinghua Univ, State Key Lab Biomembrane & Membrane Biotechnol, Struct Biol Ctr, Sch Life Sci, Beijing 100084, Peoples R China.
中文摘要	FocA is a representative member of the formate-nitrite transporter family, which transports short-chain acids in bacteria, archaea, fungi, algae and parasites. The structure and transport mechanism of the formate-nitrite transporter family remain unknown. Here we report the crystal structure of Escherichia coli FocA at 2.25 angstrom resolution. FocA forms a symmetric pentamer, with each protomer consisting of six transmembrane segments. Despite a lack of sequence homology, the overall structure of the FocA protomer closely resembles that of aquaporin and strongly argues that FocA is a channel, rather than a transporter. Structural analysis identifies potentially important channel residues, defines the channel path and reveals two constriction sites. Unlike aquaporin, FocA is impermeable to water but allows the passage of formate. A structural and biochemical investigation provides mechanistic insights into the channel activity of FocA.
收录类别	SCI
资助信息	Ministry of Science and Technology of China [2009CB918801, 2009CB918802]; National Natural Science Foundation of China [30888001]; Beijing Municipal Commissions of Education and Science and Technology; Yuyuan Foundation; Li's Foundation
语种	英语
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://ir.iphy.ac.cn/handle/311004/53599]
专题	物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文
推荐引用方式 GB/T 7714	Wang, Y,Huang, YJ,Wang, JW,et al. Structure of the formate transporter FocA reveals a pentameric aquaporin-like channel[J]. NATURE,2009,462(7272):467.
APA	Wang, Y.,Huang, YJ.,Wang, JW.,Cheng, C.,Huang, WJ.,...&Shi, YG.(2009).Structure of the formate transporter FocA reveals a pentameric aquaporin-like channel.NATURE,462(7272),467.
MLA	Wang, Y,et al."Structure of the formate transporter FocA reveals a pentameric aquaporin-like channel".NATURE 462.7272(2009):467.