Concentration control of carbon nanotubes in aqueous solution and its influence on the growth behavior of fibroblasts

CORC > 物理研究所 > 物理所公开发表论文 > 期刊论文

	Concentration control of carbon nanotubes in aqueous solution and its influence on the growth behavior of fibroblasts
	Meng, J ; Yang, M ; Song, L ; Kong, H ; Wang, CY ; Wang, R ; Wang, C ; Xie, SS ; Xu, HY
刊名	COLLOIDS AND SURFACES B-BIOINTERFACES
	2009
卷号	71 期号:1 页码:148
关键词	IN-VIVO TRANSPORTERS DNA CYTOTOXICITY CELLS FUNCTIONALIZATION DELIVERY TISSUE MICE
ISSN号	0927-7765
通讯作者	Xu, HY: Chinese Acad Med Sci, Inst Basic Med Sci, Beijing 100005, Peoples R China.
中文摘要	This work investigated the biological influence of water-soluble multiwalled carbon nanotubes (wsMWCNTs) on fibroblast cell growth as a function of concentration control in an aqueous solution. The wsMWCNTs were prepared by an optimal procedure of ultrasonication/concentrated acids oxidation. The concentration of wsMWCNT in the solution was quantified by an established calibration line. A stable concentration of 0.3 mg/ml was obtained in the surfactant-free water. The physicochemical properties of wsMWCNTs were characterized using scanning electron microscopy (SEM), X-ray photoelectron spectroscopy(XPS), UV/VIS/NIR spectroscopy, and dynamic light scattering (DLS). Cell proliferation and the cell cycle were examined by MTS assay. flow cytometry and TEM respectively. Experimental results showed that the oxidation degree was a key factor that determined the concentration and stability of wsMWCNTs in the aqueous Solution. The wsMWCNTs were able to enter into the cells and mainly accumulated in the cytoplasm. The wsMWCNTs-induced variations in cell proliferation and the cell cycle were concentration dependent. Cells cultivated with wsMWCNTs of 0.3 mg/ml underwent a dramatic apoptosis. The proliferation was clearly Suppressed when the cells were cultivated with wsMWCNTs of 0.03 mg/ml. There were no obvious influences on cell proliferation and the cell cycle when the concentration of wsMWNTs decreased to 0.01 mg/ml. (C) 2009 Elsevier B.V. All rights reserved.
收录类别	SCI
资助信息	Natural Science Foundation of China [90306004]; National Center of Nanoscience and Technology of China; National Key Scientific Projects of China [2006CB933203]
语种	英语
公开日期	2013-09-17
内容类型	期刊论文
源URL	[http://ir.iphy.ac.cn/handle/311004/35028]
专题	物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文
推荐引用方式 GB/T 7714	Meng, J,Yang, M,Song, L,et al. Concentration control of carbon nanotubes in aqueous solution and its influence on the growth behavior of fibroblasts[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2009,71(1):148.
APA	Meng, J.,Yang, M.,Song, L.,Kong, H.,Wang, CY.,...&Xu, HY.(2009).Concentration control of carbon nanotubes in aqueous solution and its influence on the growth behavior of fibroblasts.COLLOIDS AND SURFACES B-BIOINTERFACES,71(1),148.
MLA	Meng, J,et al."Concentration control of carbon nanotubes in aqueous solution and its influence on the growth behavior of fibroblasts".COLLOIDS AND SURFACES B-BIOINTERFACES 71.1(2009):148.