A novel function for the cellulose binding module of cellobiohydrolase I

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	A novel function for the cellulose binding module of cellobiohydrolase I
	Wang, LS ; Zhang, YZ ; Gao, PJ
刊名	SCIENCE IN CHINA SERIES C-LIFE SCIENCES
	2008
卷号	51 期号:7 页码:620
关键词	TRICHODERMA-PSEUDOKONINGII S-38 MOLECULAR-DYNAMICS SIMULATION NEUTRON FIBER DIFFRACTION NON-HYDROLYTIC DISRUPTION ATOMIC-FORCE MICROSCOPY HYDROGEN-BONDING SYSTEM SYNCHROTRON X-RAY CRYSTALLINE CELLULOSE MICROCRYSTALLINE CELLULOSE INDUCED FIT
ISSN号	1006-9305
通讯作者	Gao, PJ: Shandong Univ, State Key Lab Microbial Technol, Jinan 250100, Peoples R China.
中文摘要	A homogeneous cellulose-binding module (CBM) of cellobiohydrolase I (CBHI) from Trichoderma pseudokoningii S-38 was obtained by the limited proteolysis with papain and a series of chromatographs filtration. Analysis of FT-IR spectra demonstrated that the structural changes result from a weakening and splitting of the hydrogen bond network in cellulose by the action of CBM(CBHI) at 40C for 24 h. The results of molecular dynamic simulations are consistent with the experimental conclusions, and provide a nanoscopic view of the mechanism that strong and medium H-bonds decreased dramatically when CBM was bound to the cellulose surface. The function of CBM(CBHI) is not only limited to locating intact CBHI in close proximity with cellulose fibrils, but also is involved in the structural disruption at the fibre surface. The present studies provided considerable evidence for the model of the intramolecular synergy between the catalytic domain and their CBMs.
收录类别	SCI
语种	英语
公开日期	2013-09-17
内容类型	期刊论文
源URL	[http://ir.iphy.ac.cn/handle/311004/33130]
专题	物理研究所_物理所公开发表论文_物理所公开发表论文_期刊论文
推荐引用方式 GB/T 7714	Wang, LS,Zhang, YZ,Gao, PJ. A novel function for the cellulose binding module of cellobiohydrolase I[J]. SCIENCE IN CHINA SERIES C-LIFE SCIENCES,2008,51(7):620.
APA	Wang, LS,Zhang, YZ,&Gao, PJ.(2008).A novel function for the cellulose binding module of cellobiohydrolase I.SCIENCE IN CHINA SERIES C-LIFE SCIENCES,51(7),620.
MLA	Wang, LS,et al."A novel function for the cellulose binding module of cellobiohydrolase I".SCIENCE IN CHINA SERIES C-LIFE SCIENCES 51.7(2008):620.