Proteomic analysis of the seed development in Jatropha curcas: From carbon flux to the lipid accumulation

CORC > 昆明植物研究所 > 中国科学院昆明植物研究所 > 资源植物与生物技术所级重点实验室

	Proteomic analysis of the seed development in Jatropha curcas: From carbon flux to the lipid accumulation
	Liu, Hui 1,2; Wang, Cuiping 1,2; Komatsu, Setsuko 3; He, Mingxia4 ; Liu, Gongshe 1; Shen, Shihua 1
刊名	JOURNAL OF PROTEOMICS
	2013-10-08
卷号	91 页码:23-40
关键词	Jatropha curcas Proteomics Seed development Carbon flux Lipid accumulation
ISSN号	1874-3919
通讯作者	Liu,GS (reprint author),Chinese Acad Sci,Inst Bot,Key Lab Res & Dev Resource Plant,Beijing 100093,Peoples R China.,liugs@ibcas.ac.cn ; shshen@ibcas.ac.cn
英文摘要	To characterize the metabolic signatures of lipid accumulation in Jatropha curcas seeds, comparative proteomic technique was employed to Profile protein changes during the seed development. Temporal changes in comparative proteome were examined using gels-based proteomic technique at six developmental stages for lipid accumulation. And 104 differentially expressed proteins were identified by MALDI-TOF/TOF tandem mass spectrometry. These protein species were classified into 10 functional categories, and the results demonstrated that protein species related to energy and metabolism were notably accumulated and involved in the carbon flux to lipid accumulation that occurs primarily from early to late stage in seed development. Glycolysis and oxidative pentose phosphate pathways were the major pathways of producing carbon flux, and the glucose-6-phosphate and triose-phosphate are the major carbon source for fatty acid synthesis. Lipid analysis revealed that fatty acid accumulation initiated 25 days after flowering at the late stage of seed development of J. curcas. Furthermore, C-16:0 was initially synthesized as the precursor for the elongation to C-18:1 and C-18:2 in the developing seeds of J. curcas. Together, the metabolic signatures on protein changes in seed development provide profound knowledge and perspective insights into understanding lipid network in J. curcas.
学科主题	Biochemistry & Molecular Biology
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemical Research Methods
研究领域[WOS]	Biochemistry & Molecular Biology
关键词[WOS]	FATTY-ACID SYNTHESIS ; CARRIER-PROTEIN DESATURASE ; 2-DIMENSIONAL GEL-ELECTROPHORESIS ; ACETYL-COENZYME-A ; TRANSCRIPTOME ANALYSIS ; MALATE-DEHYDROGENASE ; BRASSICA-NAPUS ; SYNTHASE-III ; OIL BODIES ; PLANT
收录类别	SCI
资助信息	National Natural Science Foundation of China [31270653];Knowledge Innovation Program of the Chinese Academy of Sciences [KSCX2-YW-G-035]
语种	英语
WOS记录号	WOS:000327906000003
公开日期	2014-01-13
内容类型	期刊论文
源URL	[http://ir.kib.ac.cn/handle/151853/17437]
专题	昆明植物研究所_资源植物与生物技术所级重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Res & Dev Resource Plant, Beijing 100093, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Natl Inst Crop Sci, Tsukuba, Ibaraki 3058518, Japan 4.Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Peoples R China
推荐引用方式 GB/T 7714	Liu, Hui,Wang, Cuiping,Komatsu, Setsuko,et al. Proteomic analysis of the seed development in Jatropha curcas: From carbon flux to the lipid accumulation[J]. JOURNAL OF PROTEOMICS,2013,91:23-40.
APA	Liu, Hui,Wang, Cuiping,Komatsu, Setsuko,He, Mingxia,Liu, Gongshe,&Shen, Shihua.(2013).Proteomic analysis of the seed development in Jatropha curcas: From carbon flux to the lipid accumulation.JOURNAL OF PROTEOMICS,91,23-40.
MLA	Liu, Hui,et al."Proteomic analysis of the seed development in Jatropha curcas: From carbon flux to the lipid accumulation".JOURNAL OF PROTEOMICS 91(2013):23-40.