Microsatellite-centromere mapping in bighead carp (Aristichthys nobilis) using gynogenetic diploid families

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	Microsatellite-centromere mapping in bighead carp (Aristichthys nobilis) using gynogenetic diploid families
	Liu, Lusha 1,2; Tong, Jingou 1; Guo, Wenjie 1,2; Yu, Xiaomu 1
刊名	AQUACULTURE RESEARCH
	2013-08-01
卷号	44 期号:9 页码:1470-1488
关键词	gynogenesis half-tetrad analysis microsatellites gene-centromere mapping bighead carp (Aristichthys nobilis)
ISSN号	1355-557X
通讯作者	Tong, JO (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China.
中文摘要	Gene-centromere mapping using half-tetrad analysis is a powerful tool for understanding chromosomal behaviour and determining the position of centromeres in relation to genes or markers in fish. In this study, eight gynogenetic diploid families induced by inhibiting of the second meiotic division were genotyped at 66 microsatellite loci for microsatellite-centromere (M-C) mapping in bighead carp (Aristichthys nobilis). The absence of paternal alleles verified the success of gynogenetic development in all gynogenetic families. All loci were consistent with Mendelian segregation in control families. The estimated recombination frequency (y) ranged from 0.057 to 0.875 with an average of 0.477 +/- 0.222. Seventeen loci (25.76%) showed high M-C recombination frequencies of over 0.667. M-C distances ranged from 2.85 to 43.75cM under the assumption of complete interference. Thus, these loci are distributed from the centromeres to the telomeres of their respective chromosomes, while mainly in the intermediate region. Information on centromere mapping could serve as a starting point to consolidate the genetic linkage groups in bighead carp.
英文摘要	Gene-centromere mapping using half-tetrad analysis is a powerful tool for understanding chromosomal behaviour and determining the position of centromeres in relation to genes or markers in fish. In this study, eight gynogenetic diploid families induced by inhibiting of the second meiotic division were genotyped at 66 microsatellite loci for microsatellite-centromere (M-C) mapping in bighead carp (Aristichthys nobilis). The absence of paternal alleles verified the success of gynogenetic development in all gynogenetic families. All loci were consistent with Mendelian segregation in control families. The estimated recombination frequency (y) ranged from 0.057 to 0.875 with an average of 0.477 +/- 0.222. Seventeen loci (25.76%) showed high M-C recombination frequencies of over 0.667. M-C distances ranged from 2.85 to 43.75cM under the assumption of complete interference. Thus, these loci are distributed from the centromeres to the telomeres of their respective chromosomes, while mainly in the intermediate region. Information on centromere mapping could serve as a starting point to consolidate the genetic linkage groups in bighead carp.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Fisheries
研究领域[WOS]	Fisheries
关键词[WOS]	HALF-TETRAD ANALYSIS ; CROSS-SPECIES APPLICATION ; ZEBRAFISH DANIO-RERIO ; HYPOPHTHALMICHTHYS-MOLITRIX ; RAINBOW-TROUT ; SEGREGATION DISTORTION ; ORYZIAS-LATIPES ; LINKAGE MAP ; DNA MARKERS ; LOCI
收录类别	SCI
资助信息	Ministry of Agriculture [200903045, 2011-G12]; Ministry of Science and Technology of China [2010CB126305]; CAS studentship
语种	英语
WOS记录号	WOS:000322814000014
公开日期	2014-01-06
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/19580]
专题	水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
作者单位	1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Liu, Lusha,Tong, Jingou,Guo, Wenjie,et al. Microsatellite-centromere mapping in bighead carp (Aristichthys nobilis) using gynogenetic diploid families[J]. AQUACULTURE RESEARCH,2013,44(9):1470-1488.
APA	Liu, Lusha,Tong, Jingou,Guo, Wenjie,&Yu, Xiaomu.(2013).Microsatellite-centromere mapping in bighead carp (Aristichthys nobilis) using gynogenetic diploid families.AQUACULTURE RESEARCH,44(9),1470-1488.
MLA	Liu, Lusha,et al."Microsatellite-centromere mapping in bighead carp (Aristichthys nobilis) using gynogenetic diploid families".AQUACULTURE RESEARCH 44.9(2013):1470-1488.