Toxic effects of microcystin-LR on the HepG2 cell line under hypoxic and normoxic conditions

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	Toxic effects of microcystin-LR on the HepG2 cell line under hypoxic and normoxic conditions
	Zhang, Xin 1,2; Xie, Ping 2; Zhang, Xuezhen 1; Zhou, Wenshan 1,2; Zhao, Sujuan 2; Zhao, Yanyan 2; Cai, Yan 2
刊名	JOURNAL OF APPLIED TOXICOLOGY
	2013-10-01
卷号	33 期号:10 页码:1180-1186
关键词	MC-LR hypoxia normoxia oxidative stress apoptosis proliferation
ISSN号	0260-437X
通讯作者	Xie, P (reprint author), Inst Hydrobiol, Donghu South Rd 7, Wuhan 430072, Peoples R China.
中文摘要	Microcystins (MCs) are highly liver-specific and evidenced as a liver tumor promoter. Oxidative stress is one of the most important toxicity mechanisms of MCs, which is tightly related to oxygen concentration. The effects of MCs on animals and cell lines in normoxia and the mechanisms have been well studied, but such effects in different oxygen conditions were still unclear. The aim of the present study was to explore the cellular response of the human hepatocellular carcinoma cell line (HepG2) to MC-LR exposure under hypoxic (1% O-2) and normoxic (21% O-2) conditions. We examined cell viability, mitochondrial membrane potential (MMP), superoxide dismutase (SOD) activity and gene expression posttoxin exposure. Cell viability was increased by MC-LR in normoxia although decreased in hypoxia. MC-LR markedly induced MMP loss under hypoxic condition but only slightly MMP loss under normoxic condition. SOD activity was significantly induced by MC-LR in hypoxia, indicating prolonged oxidative stress. Inhibitory apoptosis protein (c-IAP2) was significantly up-regulated by MC-LR under normoxic condition, suggesting that c-IAP2 played an important role in the promotion of cell proliferation by MC-LR. These results indicate that MC-LR promotes cell proliferation under normoxic condition whereas induces cell apoptosis under hypoxic condition. Copyright (C) 2012 John Wiley & Sons, Ltd.
英文摘要	Microcystins (MCs) are highly liver-specific and evidenced as a liver tumor promoter. Oxidative stress is one of the most important toxicity mechanisms of MCs, which is tightly related to oxygen concentration. The effects of MCs on animals and cell lines in normoxia and the mechanisms have been well studied, but such effects in different oxygen conditions were still unclear. The aim of the present study was to explore the cellular response of the human hepatocellular carcinoma cell line (HepG2) to MC-LR exposure under hypoxic (1% O-2) and normoxic (21% O-2) conditions. We examined cell viability, mitochondrial membrane potential (MMP), superoxide dismutase (SOD) activity and gene expression posttoxin exposure. Cell viability was increased by MC-LR in normoxia although decreased in hypoxia. MC-LR markedly induced MMP loss under hypoxic condition but only slightly MMP loss under normoxic condition. SOD activity was significantly induced by MC-LR in hypoxia, indicating prolonged oxidative stress. Inhibitory apoptosis protein (c-IAP2) was significantly up-regulated by MC-LR under normoxic condition, suggesting that c-IAP2 played an important role in the promotion of cell proliferation by MC-LR. These results indicate that MC-LR promotes cell proliferation under normoxic condition whereas induces cell apoptosis under hypoxic condition. Copyright (C) 2012 John Wiley & Sons, Ltd.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Toxicology
研究领域[WOS]	Toxicology
关键词[WOS]	INDUCED APOPTOSIS ; OXIDATIVE STRESS ; RAT HEPATOCYTES ; IN-VIVO ; OXYGEN ; LIVER ; CYANOBACTERIA ; HEPATOTOXIN ; TRANSPORT ; PATHWAY
收录类别	SCI
资助信息	China National Fundamental Fund of Personnel Training [J0730649]
语种	英语
WOS记录号	WOS:000322331900015
公开日期	2014-01-03
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/19544]
专题	水生生物研究所_淡水生态学研究中心_期刊论文
作者单位	1.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Xin,Xie, Ping,Zhang, Xuezhen,et al. Toxic effects of microcystin-LR on the HepG2 cell line under hypoxic and normoxic conditions[J]. JOURNAL OF APPLIED TOXICOLOGY,2013,33(10):1180-1186.
APA	Zhang, Xin.,Xie, Ping.,Zhang, Xuezhen.,Zhou, Wenshan.,Zhao, Sujuan.,...&Cai, Yan.(2013).Toxic effects of microcystin-LR on the HepG2 cell line under hypoxic and normoxic conditions.JOURNAL OF APPLIED TOXICOLOGY,33(10),1180-1186.
MLA	Zhang, Xin,et al."Toxic effects of microcystin-LR on the HepG2 cell line under hypoxic and normoxic conditions".JOURNAL OF APPLIED TOXICOLOGY 33.10(2013):1180-1186.