Apoptotic responses of zebrafish (Danio rerio) after exposure with microcystin-LR under different ambient temperatures

CORC > 水生生物研究所 > 中科院水生所知识产出（2009年前） > 水生生物分子与细胞生物学研究中心 > 期刊论文

	Apoptotic responses of zebrafish (Danio rerio) after exposure with microcystin-LR under different ambient temperatures
	Ji, Wei 1,2; Liang, Hualei 1; Zhou, Wenshan 1; Zhang, Xuezhen 1
刊名	JOURNAL OF APPLIED TOXICOLOGY
	2013-08-01
卷号	33 期号:8 页码:799-806
关键词	cyanobacterial toxin temperature ultrastructural changes aapoptosis real-time PCR
ISSN号	0260-437X
通讯作者	Zhang, XZ (reprint author), Huazhong Agr Univ, Coll Fisheries, Key Lab Agr Anim Genet Breeding & Reprod, Minist Educ, Wuhan 430070, Peoples R China.
中文摘要	Microcystins (MCs) can cause evident hepatic apoptosis. In vitro studies indicated that uptake of MC by isolated hepatocytes was dramatically reduced as ambient temperature dropped, and some studies presented a hypothesis that differences in core body temperatures in animals result in diverse uptake of MC, as well as different toxic effects. Thus far, however, few in vivo studies have been conducted to investigate the effects of temperature on MC-induced hepatocyte apoptosis in fish, a typical poikilotherm. In the present study, zebrafish were treated with MC-LR, an MC metabolite, at three water temperatures (12, 22 and 32 degrees C), and evident differences in apoptotic profiles were observed. Damage to liver ultrastructures revealed temperature-dependent early-stage patterns of apoptosis. Flow-cytometric analysis indicated that hepatocyte apoptotic rates varied with a temperature-dependent effect. The transcription levels of some apoptosis-related genes were determined using quantitative real-time polymerase chain reaction, and significantly elevated gene expressions of P53, Bcl-2, Bax and caspase-3 were found in the 12 and 32 degrees C groups. Results of the present study indicate that different ambient temperatures can lead to various toxic effects of MCs on hepatic apoptosis in fish. Copyright (c) 2012 John Wiley & Sons, Ltd.
英文摘要	Microcystins (MCs) can cause evident hepatic apoptosis. In vitro studies indicated that uptake of MC by isolated hepatocytes was dramatically reduced as ambient temperature dropped, and some studies presented a hypothesis that differences in core body temperatures in animals result in diverse uptake of MC, as well as different toxic effects. Thus far, however, few in vivo studies have been conducted to investigate the effects of temperature on MC-induced hepatocyte apoptosis in fish, a typical poikilotherm. In the present study, zebrafish were treated with MC-LR, an MC metabolite, at three water temperatures (12, 22 and 32 degrees C), and evident differences in apoptotic profiles were observed. Damage to liver ultrastructures revealed temperature-dependent early-stage patterns of apoptosis. Flow-cytometric analysis indicated that hepatocyte apoptotic rates varied with a temperature-dependent effect. The transcription levels of some apoptosis-related genes were determined using quantitative real-time polymerase chain reaction, and significantly elevated gene expressions of P53, Bcl-2, Bax and caspase-3 were found in the 12 and 32 degrees C groups. Results of the present study indicate that different ambient temperatures can lead to various toxic effects of MCs on hepatic apoptosis in fish. Copyright (c) 2012 John Wiley & Sons, Ltd.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Toxicology
研究领域[WOS]	Toxicology
关键词[WOS]	CARP CYPRINUS-CARPIO ; TROUT ONCORHYNCHUS-MYKISS ; PROGRAMMED CELL-DEATH ; CYCLIC PEPTIDE TOXIN ; BIOCHEMICAL-CHARACTERIZATION ; CYANOBACTERIAL TOXIN ; TUMOR-SUPPRESSOR ; RAT HEPATOCYTES ; IN-VITRO ; P53
收录类别	SCI
资助信息	National Science Foundation for Distinguished Young Scholars of Hubei Province [2010CDA095]; Fundamental Research Funds for the Central Universities [2011PY003]
语种	英语
WOS记录号	WOS:000320778400010
公开日期	2014-01-06
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/19617]
专题	水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文
作者单位	1.Huazhong Agr Univ, Coll Fisheries, Key Lab Agr Anim Genet Breeding & Reprod, Minist Educ, Wuhan 430070, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Peoples R China
推荐引用方式 GB/T 7714	Ji, Wei,Liang, Hualei,Zhou, Wenshan,et al. Apoptotic responses of zebrafish (Danio rerio) after exposure with microcystin-LR under different ambient temperatures[J]. JOURNAL OF APPLIED TOXICOLOGY,2013,33(8):799-806.
APA	Ji, Wei,Liang, Hualei,Zhou, Wenshan,&Zhang, Xuezhen.(2013).Apoptotic responses of zebrafish (Danio rerio) after exposure with microcystin-LR under different ambient temperatures.JOURNAL OF APPLIED TOXICOLOGY,33(8),799-806.
MLA	Ji, Wei,et al."Apoptotic responses of zebrafish (Danio rerio) after exposure with microcystin-LR under different ambient temperatures".JOURNAL OF APPLIED TOXICOLOGY 33.8(2013):799-806.