Combined Genomics and Experimental Analyses of Respiratory Characteristics of Shewanella putrefaciens W3-18-1

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	Combined Genomics and Experimental Analyses of Respiratory Characteristics of Shewanella putrefaciens W3-18-1
	Qiu, Dongru 1,2,3,4; Wei, Hehong 3,4; Tu, Qichao 1,2; Yang, Yunfeng 5,6; Xie, Ming 1,2; Chen, Jingrong 1,2; Pinkerton, Mark H., Jr.1,2; Liang, Yili 1,2; He, Zhili 1,2; Zhou, Jizhong 1,2
刊名	APPLIED AND ENVIRONMENTAL MICROBIOLOGY
	2013-09-01
卷号	79 期号:17 页码:5250-5257
关键词	C-TYPE CYTOCHROME PERIPLASMIC NITRATE REDUCTASE ONEIDENSIS MR-1 ANAEROBIC RESPIRATION FUMARATE REDUCTASE ESCHERICHIA-COLI PSEUDOMONAS-AERUGINOSA GENUS SHEWANELLA SYSTEMS BIOLOGY SP. NOV
ISSN号	0099-2240
通讯作者	Zhou, JZ (reprint author), Univ Oklahoma, Inst Environm Genom, Norman, OK 73019 USA.
中文摘要	It has previously been shown that the Shewanella putrefaciens W3-18-1 strain produces remarkably high current in microbial fuel cells (MFCs) and can form magnetite at 0 degrees C. To explore the underlying mechanisms, we developed a genetic manipulation method by deleting the restriction-modification system genes of the SGI1 (Salmonella genome island 1)-like prophage and analyzed the key genes involved in bacterial respiration. W3-18-1 has less respiratory flexibility than the well-characterized S. oneidensis MR-1 strain, as it possesses fewer cytochrome c genes and lacks the ability to oxidize sulfite or reduce dimethyl sulfoxide (DMSO) and timethylamine oxide (TMAO). W3-18-1 lacks the hydrogen-producing Fe-only hydrogenase, and the hydrogen-oxidizing Ni-Fe hydrogenase genes were split into two separate clusters. Two periplasmic nitrate reductases (NapDAGHB and NapDABC) were functionally redundant in anaerobic growth of W3-18-1 with nitrate as the electron acceptor, though napDABC was not regulated by Crp. Moreover, nitrate respiration started earlier in W3-18-1 than in MR-1 (with NapDAGHB only) under microoxic conditions. These results indicate that Shewanella putrefaciens W3-18-1 is well adapted to habitats with higher oxygen levels. Taken together, the results of this study provide valuable insights into bacterial genome evolution.
英文摘要	It has previously been shown that the Shewanella putrefaciens W3-18-1 strain produces remarkably high current in microbial fuel cells (MFCs) and can form magnetite at 0 degrees C. To explore the underlying mechanisms, we developed a genetic manipulation method by deleting the restriction-modification system genes of the SGI1 (Salmonella genome island 1)-like prophage and analyzed the key genes involved in bacterial respiration. W3-18-1 has less respiratory flexibility than the well-characterized S. oneidensis MR-1 strain, as it possesses fewer cytochrome c genes and lacks the ability to oxidize sulfite or reduce dimethyl sulfoxide (DMSO) and timethylamine oxide (TMAO). W3-18-1 lacks the hydrogen-producing Fe-only hydrogenase, and the hydrogen-oxidizing Ni-Fe hydrogenase genes were split into two separate clusters. Two periplasmic nitrate reductases (NapDAGHB and NapDABC) were functionally redundant in anaerobic growth of W3-18-1 with nitrate as the electron acceptor, though napDABC was not regulated by Crp. Moreover, nitrate respiration started earlier in W3-18-1 than in MR-1 (with NapDAGHB only) under microoxic conditions. These results indicate that Shewanella putrefaciens W3-18-1 is well adapted to habitats with higher oxygen levels. Taken together, the results of this study provide valuable insights into bacterial genome evolution.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology ; Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology ; Microbiology
关键词[WOS]	C-TYPE CYTOCHROME ; PERIPLASMIC NITRATE REDUCTASE ; ONEIDENSIS MR-1 ; ANAEROBIC RESPIRATION ; FUMARATE REDUCTASE ; ESCHERICHIA-COLI ; PSEUDOMONAS-AERUGINOSA ; GENUS SHEWANELLA ; SYSTEMS BIOLOGY ; SP. NOV
收录类别	SCI
资助信息	U.S. Department of Energy (DOE) [DE-FG02-07ER64383]; Chinese Academy of Science [Y15103-1-401]; ENIGMA under the Office of Science, Office of Biological and Environmental Research, of the U.S. Department of Energy [DE-AC02-05CH11231]
语种	英语
WOS记录号	WOS:000322828100019
公开日期	2014-01-06
内容类型	期刊论文
源URL	[http://ir.ihb.ac.cn/handle/342005/19593]
专题	水生生物研究所_水环境工程研究中心_期刊论文
作者单位	1.Univ Oklahoma, Inst Environm Genom, Norman, OK 73019 USA 2.Univ Oklahoma, Dept Microbiol & Plant Biol, Norman, OK 73019 USA 3.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Peoples R China 4.Chinese Acad Sci, Grad Univ, Wuhan, Peoples R China 5.Tsinghua Univ, Sch Environm, State Key Joint Lab Environm Simulat & Pollut Con, Beijing 100084, Peoples R China 6.Univ Calif Berkeley, Lawrence Berkeley Natl Lab, Div Earth Sci, Berkeley, CA 94720 USA
推荐引用方式 GB/T 7714	Qiu, Dongru,Wei, Hehong,Tu, Qichao,et al. Combined Genomics and Experimental Analyses of Respiratory Characteristics of Shewanella putrefaciens W3-18-1[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2013,79(17):5250-5257.
APA	Qiu, Dongru.,Wei, Hehong.,Tu, Qichao.,Yang, Yunfeng.,Xie, Ming.,...&Zhou, Jizhong.(2013).Combined Genomics and Experimental Analyses of Respiratory Characteristics of Shewanella putrefaciens W3-18-1.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,79(17),5250-5257.
MLA	Qiu, Dongru,et al."Combined Genomics and Experimental Analyses of Respiratory Characteristics of Shewanella putrefaciens W3-18-1".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 79.17(2013):5250-5257.