Carbon nanotubes reinforced hollow fiber solid phase microextraction for the determination of strychnine and brucine in urine

CORC > 兰州化学物理研究所 > 中国科学院兰州化学物理研究所 > 中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室

	Carbon nanotubes reinforced hollow fiber solid phase microextraction for the determination of strychnine and brucine in urine
	Song XY(宋昕玥)1,2,3 ; Shi YP(师彦平)1,2 ; Chen J(陈娟)1,2 ; Chen J(陈娟)
刊名	Talanta
	2013
卷号	116 页码:188-194
关键词	Multi-walled carbon nanotubes Hollow fiber Solid-phase microextraction Surfactant Strychnine Brucine
ISSN号	0039-9140
通讯作者	陈娟
英文摘要	A mixed matrix membrane (MMM), based on carbon nanotubes (CNTs) and hollow fiber (HF), was prepared and combined with solid phase microextraction (SPME) mode to determine strychnine and brucine in urine. This MMM was prepared by dispersing CNTs in water via surfactant assistance, and then immobilizing CNTs into the pores of HF by capillary forces and sonification. The prepared carbon nanotubes reinforced hollow fiber (CNTs–HF) was subsequently wetted by a few microliters of organic solvent (1-octanol), and then applied to extract the target analytes in direct immersion sampling mode. After extraction, analytes were desorbed via ultrasonic-assisted effect, and then detected via highperformance liquid chromatography (HPLC). To achieve the highest extraction efficiency, main extraction parameters such as the type and amount of surfactant, the diameter and doping level of CNTs, extraction time, desorption condition, pH value, stirring rate and volume of the donor phase were optimized. Under the optimum extraction conditions, the method showed good linearity ranges with correlation coefficients higher than 0.9990, good repeatability and batch-to-batch reproducibility with relative standard deviations (RSDs) less than 6% and 5% for strychnine and brucine, respectively, and low limits of detection (0.7 and 0.9 μg L⁻¹ for strychnine and brucine, respectively). The recoveries were in the range of 83.81–116.14% at three spiked levels. The developed method was successfully applied to real urine sample with mean relative recoveries of 94.28% and 91.30% for strychnine and brucine, respectively. The developed method shows comparable results against reference methods and is a simple, green, and cost-effective microextraction technique.
学科主题	分析化学与药物化学
收录类别	SCI
资助信息	the National Nature Science Foundation of China (Nos. 21105106;21075127);Natural Science Foundation of Gansu Province (No.1107RJZA146)
语种	英语
WOS记录号	WOS:000328176000028
公开日期	2013-12-11
内容类型	期刊论文
源URL	[http://210.77.64.217/handle/362003/4636]
专题	兰州化学物理研究所_中科院西北特色植物资源化学重点实验室/甘肃省天然药物重点实验室
通讯作者	Chen J(陈娟)
作者单位	1.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Chem Northwestern Plant Resources, Lanzhou 730000, Peoples R China 2.Chinese Acad Sci, Lanzhou Inst Chem Phys, Key Lab Nat Med Gansu Prov, Lanzhou 730000, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Song XY,Shi YP,Chen J,et al. Carbon nanotubes reinforced hollow fiber solid phase microextraction for the determination of strychnine and brucine in urine[J]. Talanta,2013,116:188-194.
APA	Song XY,Shi YP,Chen J,&陈娟.(2013).Carbon nanotubes reinforced hollow fiber solid phase microextraction for the determination of strychnine and brucine in urine.Talanta,116,188-194.
MLA	Song XY,et al."Carbon nanotubes reinforced hollow fiber solid phase microextraction for the determination of strychnine and brucine in urine".Talanta 116(2013):188-194.