Application of Three-Dimension Printing Nano-Carbonated-Hydroxylapatite to the Repair of Defects in Rabbit Bone

doi:10.2147/IJN.S439775

	Application of Three-Dimension Printing Nano-Carbonated-Hydroxylapatite to the Repair of Defects in Rabbit Bone
	Wang, Shujie 5,6,7; Shao, Chunyan 5,6; Zhao, Xingkai 7; Guo, Yizhe 7; Song, Houhui 5,6; Shen, Lida 4; Zhou, Zhenlei 7; Li, Zhen 1,2,3
刊名	INTERNATIONAL JOURNAL OF NANOMEDICINE
	2024
卷号	19 页码:15
关键词	bone 3D printing hydroxylapatite lutein repair
ISSN号	1178-2013
DOI	10.2147/IJN.S439775
通讯作者	Zhou, Zhenlei(zhouzl@njau.edu.cn) ; Li, Zhen(lizhen@njau.edu.cn)
英文摘要	Introduction: Hydroxylapatite (HAp) is a biodegradable bone graft material with high biocompatibility. However, the clinical application of HAp has been limited due to the poor absorption rate in vivo. Methods: In this study, carbonated hydroxylapatite (CHAp) with a chemical composition similar to natural bone was synthesized. HAp and CHAp scaffolds were fabricated by 3D printing. Each material was designed by two types of scaffold model with a maximum width of 8 mm and a thickness of 2 mm, ie, structure I (round shape) and structure II (grid shape). Then, the HAp scaffolds were loaded with lutein. These scaffolds were implanted into the 8 mm bone defect on the top of the rabbit skull within 3 hours in the morning. The curative effects of the scaffolds were assessed two months after implantation. Results: The 3D printed scaffolds did not cause severe inflammation or rejection after implantation. It showed that the porous structures allow bone cells to enter into the scaffolds. Furthermore, CHAp scaffolds were more biocompatible than HAp scaffolds, and showed a higher level of degradation and new bone formation after implantation. Structure II scaffolds with a smaller mineral content degraded faster than structure I, while structure I had better osteoconductive properties than structure II. Besides, the addition of lutein significantly enhanced the rate of new bone formation. Discussion: The uniqueness of this study lies in the synthesis of 3D printed CHAp scaffolds and the implantation of CHAp in rabbit bone defects. The incorporation of suitable carbonate and lutein into HAp can enhance the osteoinductivity of the graft, and CHAp has a faster degradation rate in vivo, all of which provide a new reference for the research and application of apatite-based composites.
资助项目	National Natural Science Foundation of China[32072936] ; State Key Laboratory of Palaeobiology and Stratigraphy (Nanjing Institute of Geology and Palaeontology, CAS)[223116]
WOS关键词	BETA-CRYPTOXANTHIN ; TISSUE ; FRACTURE ; BIOMATERIALS ; SUBSTITUTES ; HYDROXYAPATITE ; CAROTENOIDS ; RESORPTION ; GRAFTS ; MODELS
WOS研究方向	Science & Technology - Other Topics ; Pharmacology & Pharmacy
语种	英语
出版者	DOVE MEDICAL PRESS LTD
WOS记录号	WOS:001167703400001
资助机构	National Natural Science Foundation of China ; State Key Laboratory of Palaeobiology and Stratigraphy (Nanjing Institute of Geology and Palaeontology, CAS)
内容类型	期刊论文
源URL	[http://ir.nigpas.ac.cn/handle/332004/43427]
专题	中国科学院南京地质古生物研究所
通讯作者	Zhou, Zhenlei; Li, Zhen
作者单位	1.Chinese Acad Sci, Ctr Excellence Life & Paleoenvironm, Nanjing 210008, Jiangsu, Peoples R China 2.Chinese Acad Sci, State Key Lab Palaeobiol & Stratig, Nanjing Inst Geol & Palaeontol, Nanjing 210008, Jiangsu, Peoples R China 3.Nanjing Agr Univ, Coll Resources & Environm Sci, Nanjing 210095, Jiangsu, Peoples R China 4.Nanjing Univ Aeronaut & Astronaut, Coll Mech & Elect Engn, Nanjing 210016, Jiangsu, Peoples R China 5.Zhejiang Agr & Forestry Univ, Coll Vet Med, Hangzhou 311300, Zhejiang, Peoples R China 6.Zhejiang Agr & Forestry Univ, Coll Anim Sci & Technol, Hangzhou 311300, Zhejiang, Peoples R China 7.Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Jiangsu, Peoples R China
推荐引用方式 GB/T 7714	Wang, Shujie,Shao, Chunyan,Zhao, Xingkai,et al. Application of Three-Dimension Printing Nano-Carbonated-Hydroxylapatite to the Repair of Defects in Rabbit Bone[J]. INTERNATIONAL JOURNAL OF NANOMEDICINE,2024,19:15.
APA	Wang, Shujie.,Shao, Chunyan.,Zhao, Xingkai.,Guo, Yizhe.,Song, Houhui.,...&Li, Zhen.(2024).Application of Three-Dimension Printing Nano-Carbonated-Hydroxylapatite to the Repair of Defects in Rabbit Bone.INTERNATIONAL JOURNAL OF NANOMEDICINE,19,15.
MLA	Wang, Shujie,et al."Application of Three-Dimension Printing Nano-Carbonated-Hydroxylapatite to the Repair of Defects in Rabbit Bone".INTERNATIONAL JOURNAL OF NANOMEDICINE 19(2024):15.