Metabolic proteins with crucial roles in <i>Edwardsiella tarda</i> antioxidative adaptation and intracellular proliferation

doi:10.1128/msystems.00391-23

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	*Metabolic proteins with crucial roles in Edwardsiella tarda* antioxidative adaptation and intracellular proliferation**
	Wang, Xinhui 1,3; Sun, Boguang 1,2,3
刊名	MSYSTEMS
	2023-09-20
页码	15
关键词	Edwardsiella tarda oxidative stress virulence metabolic reprogramming proteomics
ISSN号	2379-5077
DOI	10.1128/msystems.00391-23
通讯作者	Sun, Boguang(sunboguang@qdio.ac.cn)
英文摘要	Edwardsiella tarda is a severe fish pathogen, featured by its capacity to live inside host phagocytes. For intracellular survival, it is crucial for E. tarda to neutralize the deleterious effect of host reactive oxygen species (ROS). Accumulating evidence suggests that bacterial metabolism is closely connected to oxidative resistance. However, the roles of E. tarda metabolic proteins in antioxidative adaptation and intracellular proliferation remain elusive. In this study, we performed a proteomic analysis on E. tarda and identified 111 proteins responsive to H2O2-mediated oxidative stress. Based on this data, we further obtained eight crucial proteins, including seven metabolic proteins, for E. tarda antioxidation and intracellular infection. Among them, two C4-dicarboxylate transporters were found necessary for E. tarda to disseminate in fish tissues. Furthermore, the substrate of the two transporters was identified as L-aspartate, which was proven to be essential for the full antioxidative capacity of E. tarda. Our results indicate that reprogramming the metabolic flux to the production of pyruvate, a ketoacid capable of neutralizing ROS, was likely a pivotal strategy of E. tarda to survive the oxidative environments inside host cells. Together, the findings of this study highlight the significance of metabolic reprogramming for bacterial redox homeostasis and intracellular infection.
资助项目	The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
WOS关键词	OXIDATIVE STRESS ; HYDROGEN-PEROXIDE ; PHAGOCYTES ; RESISTANCE ; PATHOGENESIS ; MECHANISMS ; KATB
WOS研究方向	Microbiology
语种	英语
出版者	AMER SOC MICROBIOLOGY
WOS记录号	WOS:001145055800001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/184365]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Sun, Boguang
作者单位	1.Chinese Acad Sci, Inst Oceanol, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China 2.Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China 3.Chinese Acad Sci, CAS Ctr Ocean Megasci, Qingdao, Peoples R China
推荐引用方式 GB/T 7714	Wang, Xinhui,Sun, Boguang. Metabolic proteins with crucial roles in Edwardsiella tarda antioxidative adaptation and intracellular proliferation[J]. MSYSTEMS,2023:15.
APA	Wang, Xinhui,&Sun, Boguang.(2023).Metabolic proteins with crucial roles in Edwardsiella tarda antioxidative adaptation and intracellular proliferation.MSYSTEMS,15.
MLA	Wang, Xinhui,et al."Metabolic proteins with crucial roles in Edwardsiella tarda antioxidative adaptation and intracellular proliferation".MSYSTEMS (2023):15.