Glial cell line-derived neurotrophic factor (GDNF) is essential for colonization and expansion of turbot (Scophthalmus maximus) germ cells in recipients and in vitro culture

doi:10.1016/j.theriogenology.2023.09.013

CORC > 海洋研究所 > 中国科学院海洋研究所 > 实验海洋生物学重点实验室

	Glial cell line-derived neurotrophic factor (GDNF) is essential for colonization and expansion of turbot (Scophthalmus maximus) germ cells in recipients and in vitro culture
	Duan, Lei 1,2,3; Du, Shuran 1,3; Wang, Xueying 1,3; Zhou, Li 1,3; Liu, Qinghua 1,3; Li, Jun 1,3
刊名	THERIOGENOLOGY
	2024-01-15
卷号	214 页码:1-9
关键词	GDNF Germ cells Germ cell transplantation Turbot
ISSN号	0093-691X
DOI	10.1016/j.theriogenology.2023.09.013
通讯作者	Liu, Qinghua(qinghualiu@qdio.ac.cn) ; Li, Jun(junli@qdio.ac.cn)
英文摘要	Germ cell transplantation (GCT) is a promising biotechnology that enables the production of donor-derived gametes in surrogate recipients. It plays a crucial role in the protection of endangered species, the propaga-tion of elite species with desired traits, and long-term preservation of genetic resources. This significance is particularly pronounced when GCT is synergistically employed with cryopreservation techniques. However, GCT often encounters challenges due to low colonization rates and, in some cases, complete loss of donor cells in recipients. Glial cell line-derived neurotrophic factor (GDNF) plays a pivotal role in sustaining the self-renewal of spermatogonial stem cells (SSCs) in mammals. Additionally, it has been shown to promote the proliferation of spermatogonia in vitro cultures in certain animal species. In turbot (Scophthalmus maximus), we found that the expressions of gdnf and gfr alpha 1a were predominantly observed in spermatogonia rather than somatic cells, which differed from their expression patterns in mammals. The efficiency of exogenous spermatogonia transplantation in Japanese flounder (Paralichthys olivaceus) larvae could be substantially enhanced by incubating donor cells from turbot with 100 ng/ml GDNF prior to transplantation. This led to a noteworthy increase in the colonization rate, rising from 33%-50%-61.5%. Additionally, the addition of 20 ng/ml GDNF in cell medium could also promote the proliferation of turbot germ cells in vitro. These results demonstrated the gdnf in turbot testis expression characteristics and suggested that addition of GNDF could be an effective way to improve the GCT efficiency and promote the germ cells expansion during in vitro culture.
资助项目	Shandong Province Natural Science Foundation[ZR2020KC038] ; Research and Development Program of Shandong Province[2021LZGC029] ; China Agriculture Research System[CARS-47]
WOS关键词	SPERMATOGONIAL STEM-CELLS ; YELLOWTAIL SERIOLA-QUINQUERADIATA ; RAINBOW-TROUT ; PROLIFERATION ; TRANSPLANTATION ; MAINTENANCE ; GENERATION ; SURROGATE ; SURVIVAL ; TESTIS
WOS研究方向	Reproductive Biology ; Veterinary Sciences
语种	英语
出版者	ELSEVIER SCIENCE INC
WOS记录号	WOS:001098779600001
内容类型	期刊论文
源URL	[http://ir.qdio.ac.cn/handle/337002/183849]
专题	海洋研究所_实验海洋生物学重点实验室
通讯作者	Liu, Qinghua; Li, Jun
作者单位	1.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao 266071, Peoples R China
推荐引用方式 GB/T 7714	Duan, Lei,Du, Shuran,Wang, Xueying,et al. Glial cell line-derived neurotrophic factor (GDNF) is essential for colonization and expansion of turbot (Scophthalmus maximus) germ cells in recipients and in vitro culture[J]. THERIOGENOLOGY,2024,214:1-9.
APA	Duan, Lei,Du, Shuran,Wang, Xueying,Zhou, Li,Liu, Qinghua,&Li, Jun.(2024).Glial cell line-derived neurotrophic factor (GDNF) is essential for colonization and expansion of turbot (Scophthalmus maximus) germ cells in recipients and in vitro culture.THERIOGENOLOGY,214,1-9.
MLA	Duan, Lei,et al."Glial cell line-derived neurotrophic factor (GDNF) is essential for colonization and expansion of turbot (Scophthalmus maximus) germ cells in recipients and in vitro culture".THERIOGENOLOGY 214(2024):1-9.