雨生红球藻蓝光受体向光素(PHOT)互作蛋白鉴定及烟草异源表达的生理效应

doi:10.13592/j.cnki.ppj.100547

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 海岸带生物学与生物资源利用重点实验室

	雨生红球藻蓝光受体向光素(PHOT)互作蛋白鉴定及烟草异源表达的生理效应
	李旺宁 2; 李亚男 2; 梁梦静 2; 张春辉 2; 季春丽 2; 李润植 2; 崔玉琳 1; 秦松 1; 薛金爱 2; 崔红利 1,2
刊名	植物生理学报
	2023
卷号	59 期号:7 页码:1300-1310
关键词	雨生红球藻(Haematococcus pluvialis) 向光素(PHOT) 互作蛋白烟草异源表达生理效应
ISSN号	2095-1108
DOI	10.13592/j.cnki.ppj.100547
其他题名	Identification of blue light receptor phototropin (PHOT) interacting protein from Haematococcus pluvialis and physiological effect of its heterologous expression in tobacco
文献子类	期刊论文
英文摘要	Photropin (PHOT) is a unique blue light receptor in plants and green algae.Identification of PHOT interacting proteins and their functions will lay a foundation for elucidating PHOT-mediated signaling pathways and physiological functions.In this study,the yeast two-hybridization (Y2H) was used to screen the candidate interacting proteins of HaePHOT from the cDNA library of Haematococcus pluvialis.A total of 23 positive clones were obtained,of which four clones were annotated as DnaJ.The expression correlation of HaePHOT and DnaJ in H.pluvialis showed a strong positive model basing on RNA-Seq data.Further analysis of the expression correlation between HaePHOT or DnaJ and astaxanthin synthetic genes showed that HaePHOT and DnaJ were positively correlated with LYCB,BKT,BCH and CYP97B,but negatively correlated with PSY,PDS,KAS and FAD,respectively.Transient expression assay in tobacco showed that there was no significant difference in chlorophyll a,chlorophyll b and carotenoids contents between wildtype tobacco and HaePHOT-overexpressing tobacco under normal light (white light).However,under blue light,the contents of those pigments were higher in the transient-expression plants than that in the wildtype tobacco.qRT-PCR analysis showed that the expression of genes encoding chlorophyll a oxygenase(CAO),protochlorophyllide oxidoreductase (POR),phytoene synthase (PSY),phytoene desaturase (PDS) and lycopene beta-cyclase (LYCB) were significantly increased under blue light irradiation in the transient-expression tobacco plants,which was consistent with the change pattern of chlorophylls and carotenoids contents.These findings provide a theoretical basis for further revealing the regulatory mechanisms mediated by HaePHOT and its interacting proteins.
语种	中文
CSCD记录号	CSCD:7523459
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/34263]
专题	海岸带生物学与生物资源利用重点实验室
作者单位	1.中国科学院烟台海岸带研究所海岸带生物学与生物资源保护重点实验室,山东烟台264003 2.山西农业大学农学院/分子农业与生物能源研究所,山西晋中030801;
推荐引用方式 GB/T 7714	李旺宁,李亚男,梁梦静,等. 雨生红球藻蓝光受体向光素(PHOT)互作蛋白鉴定及烟草异源表达的生理效应[J]. 植物生理学报,2023,59(7):1300-1310.
APA	李旺宁.,李亚男.,梁梦静.,张春辉.,季春丽.,...&崔红利.(2023).雨生红球藻蓝光受体向光素(PHOT)互作蛋白鉴定及烟草异源表达的生理效应.植物生理学报,59(7),1300-1310.
MLA	李旺宁,et al."雨生红球藻蓝光受体向光素(PHOT)互作蛋白鉴定及烟草异源表达的生理效应".植物生理学报 59.7(2023):1300-1310.