NIR Light-Activated Mitochondrial RNA Cross-Linking Strategy for H<sub>2</sub>S Monitoring and Prolonged Colorectal Tumor Imaging

doi:10.1021/acs.analchem.3c04033

CORC > 烟台海岸带研究所 > 中国科学院烟台海岸带研究所 > 中科院海岸带环境过程与生态修复重点实验室

	NIR Light-Activated Mitochondrial RNA Cross-Linking Strategy for H₂S Monitoring and Prolonged Colorectal Tumor Imaging
	Lu, Zhihao 1,2; Tan, Jiangkun 1,3; Wu, Yuting 2; You, Jinmao 1,2; Xie, Xiunan 2; Zhang, Zhiyong 2; Li, Zan 2; Chen, Lingxin 3
刊名	ANALYTICAL CHEMISTRY
	2023-11-08
卷号	95 期号:46 页码:17089-17098
ISSN号	0003-2700
DOI	10.1021/acs.analchem.3c04033
通讯作者	You, Jinmao(jmyou6304@163.com) ; Li, Zan(lilizanzan@163.com) ; Chen, Lingxin(lxchen@yic.ac.cn)
英文摘要	Molecular diffusion and leakage impede the long-term retention of probes/drugs and may cause potential adverse effects in theranostic fields. Spatiotemporally manipulating the organelle-immobilization behavior of probes/drugs for prolonged tumor retention is indispensable to achieving effective cancer diagnosis and therapy. Herein, we propose a rational strategy that could realize near-infrared light-activated ribonucleic acids (RNAs) cross-linking for prolonged tumor retention and simultaneously endogenous hydrogen sulfide (H2S) monitoring in colorectal tumors. Profiting from efficient singlet oxygen (O-1(2)) generation from Cy796 under 808 nm light irradiation, the O-1(2)-animated furan moiety in Cy796 could covalently cross-link with cytoplasmic RNAs via a cycloaddition reaction and realize organelle immobilization. Subsequently, specific thiolysis of Cy796 assisted with H2S resulted in homologous product Cy644 with reduced O-1(2) generation yields and enhanced absolute fluorescence quantum yields (from 7.42 to 27.70%) with blue-shifted absorption and emission, which avoided the molecular oxidation fluorescence quenching effect mediated by O-1(2) and validated fluorescence imaging. Furthermore, studies have demonstrated that our proposed strategy possessed adequate capacity for fluorescence imaging and endogenous H2S detection in HCT116 cells, particularly accumulated at the tumor sites, and retained long-term imaging with excellent biocompatibility. The turn-on fluorescence mode and turn-off (1)O(2 )generation efficiency in our strategy successfully realized a diminished fluorescence cross-talk and oxidation quenching effect. It is adequately envisioned that our proposed strategy for monitoring biomarkers and prolonged tumor retention will contribute tremendous dedication in the clinical, diagnostic, and therapeutic fields.
WOS关键词	FLUORESCENT-PROBE ; HYDROGEN-SULFIDE ; LIVING CELLS ; NANOPARTICLES ; FLUCTUATIONS ; RECOGNITION ; GENERATION ; RELEASE ; DISEASE ; OXYGEN
WOS研究方向	Chemistry
语种	英语
WOS记录号	WOS:001141275900001
资助机构	National Natural Science Foundation of China ; Major innovation fund of Shandong Province ; Natural Science Foundation of Shandong Province ; Natural Science Foundation of Shandong Province
内容类型	期刊论文
源URL	[http://ir.yic.ac.cn/handle/133337/34353]
专题	烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
通讯作者	You, Jinmao; Li, Zan; Chen, Lingxin
作者单位	1.Shaoxing Univ, Coll Chem & Chem Engn, Shaoxing 312000, Peoples R China 2.Qufu Normal Univ, Sch Chem & Chem Engn, Key Lab Life Organ Anal Shandong Prov, Qufu 273165, Peoples R China 3.Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
推荐引用方式 GB/T 7714	Lu, Zhihao,Tan, Jiangkun,Wu, Yuting,et al. NIR Light-Activated Mitochondrial RNA Cross-Linking Strategy for H₂S Monitoring and Prolonged Colorectal Tumor Imaging[J]. ANALYTICAL CHEMISTRY,2023,95(46):17089-17098.
APA	Lu, Zhihao.,Tan, Jiangkun.,Wu, Yuting.,You, Jinmao.,Xie, Xiunan.,...&Chen, Lingxin.(2023).NIR Light-Activated Mitochondrial RNA Cross-Linking Strategy for H₂S Monitoring and Prolonged Colorectal Tumor Imaging.ANALYTICAL CHEMISTRY,95(46),17089-17098.
MLA	Lu, Zhihao,et al."NIR Light-Activated Mitochondrial RNA Cross-Linking Strategy for H₂S Monitoring and Prolonged Colorectal Tumor Imaging".ANALYTICAL CHEMISTRY 95.46(2023):17089-17098.