Zebularine, a DNA Methylation Inhibitor, Activates Anthocyanin Accumulation in Grapevine Cells

doi:10.3390/genes13071256

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	Zebularine, a DNA Methylation Inhibitor, Activates Anthocyanin Accumulation in Grapevine Cells
	Kong, Junhua 1,5; Garcia, Virginie ; Zehraoui, Enric ; Stammitti, Linda ; Hilbert, Ghislaine ; Renaud, Christel ; Maury, Stephane 3; Delaunay, Alain 3; Cluzet, Stephanie 4; Lecourieux, Fatma
刊名	GENES
	2022
卷号	13 期号:7
关键词	DNA methylation zebularine anthocyanin
DOI	10.3390/genes13071256
文献子类	Article
英文摘要	Through its role in the regulation of gene expression, DNA methylation can participate in the control of specialized metabolite production. We have investigated the link between DNA methylation and anthocyanin accumulation in grapevine using the hypomethylating drug, zebularine and Gamay Teinturier cell suspensions. In this model, zebularine increased anthocyanin accumulation in the light, and induced its production in the dark. To unravel the underlying mechanisms, cell transcriptome, metabolic content, and DNA methylation were analyzed. The up-regulation of stress-related genes, as well as a decrease in cell viability, revealed that zebularine affected cell integrity. Concomitantly, the global DNA methylation level was only slightly decreased in the light and not modified in the dark. However, locus-specific analyses demonstrated a decrease in DNA methylation at a few selected loci, including a CACTA DNA transposon and a small region upstream from the UFGT gene, coding for the UDP glucose:flavonoid-3-O-glucosyltransferase, known to be critical for anthocyanin biosynthesis. Moreover, this decrease was correlated with an increase in UFGT expression and in anthocyanin content. In conclusion, our data suggest that UFGT expression could be regulated through DNA methylation in Gamay Teinturier, although the functional link between changes in DNA methylation and UFGT transcription still needs to be demonstrated.
学科主题	Genetics & Heredity
电子版国际标准刊号	2073-4425
出版地	BASEL
WOS关键词	GLUCOSE-FLAVONOID 3-O-GLUCOSYLTRANSFERASE ; TRANSCRIPTION FACTOR ; PROANTHOCYANIDIN BIOSYNTHESIS ; STILBENE BIOSYNTHESIS ; CYTOSINE METHYLATION ; SUSPENSION CULTURES ; METABOLIC PATHWAYS ; GAMMA-IRRADIATION ; SYNTHASE GENES ; PLANT STRESS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	MDPI
WOS记录号	WOS:000833132100001
资助机构	China Scholarship Council
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/28848]
专题	中科院北方资源植物重点实验室
作者单位	1.Univ Bordeaux, Fac Sci Pharmaceut, Unite Rech Oenol, USC INRA 1366,ISVV,Equipe Mol Interet Biol GESVAB, F-33882 Villenave Dornon, France 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 3.Univ Bordeaux, Bordeaux Sci Agro, INRAE, UMR Ecophysiol & Genom Fonct Vigne, 210 Chemin Leysotte, F-33140 Villenave Dornon, France 4.Univ Orleans, EA1207 USC1328 Lab Biol Ligneux & Grandes Culture, INRAe, F-45067 Orleans, France 5.Chinese Acad Sci, Inst Bot, Beijing Key Lab Grape Sci & Enol, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Kong, Junhua,Garcia, Virginie,Zehraoui, Enric,et al. Zebularine, a DNA Methylation Inhibitor, Activates Anthocyanin Accumulation in Grapevine Cells[J]. GENES,2022,13(7).
APA	Kong, Junhua.,Garcia, Virginie.,Zehraoui, Enric.,Stammitti, Linda.,Hilbert, Ghislaine.,...&Gallusci, Philippe.(2022).Zebularine, a DNA Methylation Inhibitor, Activates Anthocyanin Accumulation in Grapevine Cells.GENES,13(7).
MLA	Kong, Junhua,et al."Zebularine, a DNA Methylation Inhibitor, Activates Anthocyanin Accumulation in Grapevine Cells".GENES 13.7(2022).