A dynamic phosphoproteomic analysis provides insight into the C4 plant maize (Zea mays L.) response to natural diurnal changes

doi:10.1111/tpj.16047

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院光生物学重点实验室

	A dynamic phosphoproteomic analysis provides insight into the C4 plant maize (Zea mays L.) response to natural diurnal changes
	Gao, Zhi-Fang ; Yang, Xiu ; Mei, Yingchang ; Zhang, Jiao ; Chao, Qing ; Wang, Bai-Chen
刊名	PLANT JOURNAL
	2023
卷号	113 期号:2 页码:291-307
关键词	maize C4 phosphoproteome kinase-substrate network light and temperature
ISSN号	0960-7412
DOI	10.1111/tpj.16047
文献子类	Article
英文摘要	As sessile organisms, plants need to respond to rapid changes in numerous environmental factors, mainly diurnal changes of light, temperature, and humidity. Maize is the world's most grown crop, and as a C4 plant it exhibits high photosynthesis capacity, reaching the highest rate of net photosynthesis at midday; that is, there is no midday depression. Revealing the physiological responses to diurnal changes and underlying mechanisms will be of great significance for guiding maize improvement efforts. In this study, we collected maize leaf samples and analyzed the proteome and phosphoproteome at nine time points during a single day/night cycle, quantifying 7424 proteins and 5361 phosphosites. The new phosphosites identified in our study increased the total maize phosphoproteome coverage by 8.5%. Kinase-substrate network analysis indicated that 997 potential substrates were phosphorylated by 20 activated kinases. Through analysis of proteins with significant changes in abundance and phosphorylation, we found that the response to a heat stimulus involves a change in the abundance of numerous proteins. By contrast, the high light at noon and rapidly changing light conditions induced changes in the phosphorylation level of proteins involved in processes such as chloroplast movement, photosynthesis, and C4 pathways. Phosphorylation is involved in regulating the activity of large number of enzymes; for example, phosphorylation of S55 significantly enhanced the activity of maize phosphoenolpyruvate carboxykinase1 (ZmPEPCK1). Overall, the database of dynamic protein abundance and phosphorylation we have generated provides a resource for the improvement of C4 crop plants.
学科主题	Plant Sciences
电子版国际标准刊号	1365-313X
出版地	HOBOKEN
WOS关键词	BUNDLE-SHEATH CHLOROPLASTS ; PHOTOSYSTEM-II ; PHOSPHOENOLPYRUVATE CARBOXYKINASE ; LEAF PHOTOSYNTHESIS ; REGULATORY PHOSPHORYLATION ; SUCROSE TRANSPORTER ; SEEDLING LEAVES ; HEAT-STRESS ; ARABIDOPSIS ; LIGHT
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	WILEY
WOS记录号	WOS:000898713500001
资助机构	Strategic Priority Research Program of the Chinese Academy of Sciences ; National Key Research and Development Program of China ; National Natural Science Foundation of China ; [XDA24010203] ; [2021YFA1300403] ; [31671710]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/29097]
专题	中科院光生物学重点实验室
作者单位	1.[Chao, Qing 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100093, Peoples R China 4.Inst Bot, Chinese Acad Sci, Key Lab Photobiol, CAS, Beijing 100093, Peoples R China 5.Chao, Qing 6.Zhang, Jiao 7.Mei, Yingchang 8.Yang, Xiu 9.[Gao, Zhi-Fang
推荐引用方式 GB/T 7714	Gao, Zhi-Fang,Yang, Xiu,Mei, Yingchang,et al. A dynamic phosphoproteomic analysis provides insight into the C4 plant maize (Zea mays L.) response to natural diurnal changes[J]. PLANT JOURNAL,2023,113(2):291-307.
APA	Gao, Zhi-Fang,Yang, Xiu,Mei, Yingchang,Zhang, Jiao,Chao, Qing,&Wang, Bai-Chen.(2023).A dynamic phosphoproteomic analysis provides insight into the C4 plant maize (Zea mays L.) response to natural diurnal changes.PLANT JOURNAL,113(2),291-307.
MLA	Gao, Zhi-Fang,et al."A dynamic phosphoproteomic analysis provides insight into the C4 plant maize (Zea mays L.) response to natural diurnal changes".PLANT JOURNAL 113.2(2023):291-307.