Effects of mutations of D1-R323, D1-N322, D1-D319, D1-H304 on the functioning of photosystem II in Thermosynechococcus vulcanus

doi:10.1007/s11120-022-00920-z

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	Effects of mutations of D1-R323, D1-N322, D1-D319, D1-H304 on the functioning of photosystem II in Thermosynechococcus vulcanus
	Zhu, Qingjun 2; Yang, Yanyan ; Xiao, Yanan 2; Han, Wenhui 2; Li, Xingyue 2; Wang, Wenda ; Kuang, Tingyun ; Shen, Jian-Ren ; Han, Guangye
刊名	PHOTOSYNTHESIS RESEARCH
	2022
卷号	152 期号:2 页码:193-206
关键词	Photosystem II Site-directed mutagenesis Water oxidation Hydrogen-bond networks Functional binding
ISSN号	0166-8595
DOI	10.1007/s11120-022-00920-z
文献子类	Article
英文摘要	Photosystem II (PSII) has a number of hydrogen-bonding networks connecting the manganese cluster with the lumenal bulk solution. The structure of PSII from Thermosynechococcus vulcanus (T. vulcanus) showed that D1-R323, D1-N322, D1-D319 and D1-H304 are involved in one of these hydrogen-bonding networks located in the interfaces between the D1, CP43 and PsbV subunits. In order to investigate the functions of these residues in PSII, we generated seven site-directed mutants D1-R323A, D1-R323E, D1-N322R, D1-D319L, D1-D319R, D1-D319Y and D1-H304D of T. vulcanus and examined the effects of these mutations on the growth and functions of the oxygen-evolving complex. The photoautotrophic growth rates of these mutants were similar to that of the wild type, whereas the oxygen-evolving activities of the mutant cells were decreased differently to 63-91% of that of the wild type at pH 6.5. The mutant cells showed a higher relative activity at higher pH region than the wild type cells, suggesting that higher pH facilitated proton egress in the mutants. In addition, oxygen evolution of thylakoid membranes isolated from these mutants showed an apparent decrease compared to that of the cells. This is due to the loss of PsbU during purification of the thylakoid membranes. Moreover, PsbV was also lost in the PSII core complexes purified from the mutants. Taken together, D1-R323, D1-N322, D1-D319 and D1-H304 are vital for the optimal function of oxygen evolution and functional binding of extrinsic proteins to PSII core, and may be involved in the proton egress pathway mediated by Y-Z.
学科主题	Plant Sciences
电子版国际标准刊号	1573-5079
出版地	DORDRECHT
WOS关键词	PHOTOSYNTHETIC WATER OXIDATION ; ELECTRON-TRANSFER ; MANGANESE CLUSTER ; 12-KDA PROTEIN ; PROTON RELEASE ; O2 EVOLUTION ; D1 PROTEIN ; OXYGEN ; MECHANISM ; BINDING
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000790102300001
资助机构	National Natural Science Foundation of China [31470339] ; National Key R&D Program of China [2017YFA0503700, 2020YFA0907600] ; CAS Project for Young Scientists in Basic Research [YSBR-004] ; CAS Interdisciplinary Innovation Team [JCTD-2020-06] ; Strategic Priority Research Program of CAS [XDA26050402]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/28534]
专题	中科院光生物学重点实验室
作者单位	1.Okayama Univ, Inst Interdisciplinary Sci, Grad Sch Nat Sci & Technol, Tsushima Naka 3-1-1, Okayama 7008530, Japan 2.Chinese Acad Sci, Inst Bot, Photosynth Res Ctr, Key Lab Photobiol, 20 Nanxincun, Beijing 100093, Peoples R China 3.Univ Chinese Acad Sci, Yuquan Rd, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Innovat Acad Seed Design, 1 Beichen West Rd, Beijing 100101, Peoples R China
推荐引用方式 GB/T 7714	Zhu, Qingjun,Yang, Yanyan,Xiao, Yanan,et al. Effects of mutations of D1-R323, D1-N322, D1-D319, D1-H304 on the functioning of photosystem II in Thermosynechococcus vulcanus[J]. PHOTOSYNTHESIS RESEARCH,2022,152(2):193-206.
APA	Zhu, Qingjun.,Yang, Yanyan.,Xiao, Yanan.,Han, Wenhui.,Li, Xingyue.,...&Han, Guangye.(2022).Effects of mutations of D1-R323, D1-N322, D1-D319, D1-H304 on the functioning of photosystem II in Thermosynechococcus vulcanus.PHOTOSYNTHESIS RESEARCH,152(2),193-206.
MLA	Zhu, Qingjun,et al."Effects of mutations of D1-R323, D1-N322, D1-D319, D1-H304 on the functioning of photosystem II in Thermosynechococcus vulcanus".PHOTOSYNTHESIS RESEARCH 152.2(2022):193-206.