Two chloroplast-localized MORF proteins act as chaperones to maintain tetrapyrrole biosynthesis

doi:10.1111/nph.18273

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院光生物学重点实验室

	Two chloroplast-localized MORF proteins act as chaperones to maintain tetrapyrrole biosynthesis
	Yuan, Jiarui 3; Ma, Tingting ; Ji, Shuiling 1; Hedtke, Boris 1; Grimm, Bernhard 1; Lin, Rongcheng 3
刊名	NEW PHYTOLOGIST
	2022
卷号	235 期号:5 页码:1868-1883
关键词	chaperone GUN4 MORF POR tetrapyrrole biosynthesis
ISSN号	0028-646X
DOI	10.1111/nph.18273
文献子类	Article
英文摘要	Tetrapyrroles have essential functions as pigments and cofactors during plant growth and development, and the tetrapyrrole biosynthesis pathway is tightly controlled. Multiple organellar RNA editing factors (MORFs) are required for editing of a wide variety of RNA sites in chloroplasts and mitochondria, but their biochemical properties remain elusive. Here, we uncovered the roles of chloroplast-localized MORF2 and MORF9 in modulating tetrapyrrole biosynthesis and embryogenesis in Arabidopsis thaliana. The lack or reduced transcripts of MORF2 or MORF9 significantly affected biosynthesis of the tetrapyrrole precursor 5-aminolevulinic acid and accumulation of Chl and other tetrapyrrole intermediates. MORF2 directly interacts with multiple tetrapyrrole biosynthesis enzymes and regulators, including NADPH:PROTOCHLOROPHYLLIDE OXIDOREDUCTASE B (PORB) and GENOMES UNCOUPLED4 (GUN4). Strikingly, MORF2 and MORF9 display holdase chaperone activity, alleviate the aggregation of PORB in vitro, and are essential for POR accumulation in vivo. Moreover, both MORF2 and MORF9 significantly stimulate magnesium chelatase activity. Our findings reveal a previously unknown biochemical property of MORF proteins as chaperones and point to a new layer of post-translational control of the tightly regulated tetrapyrrole biosynthesis in plants.
学科主题	Plant Sciences
电子版国际标准刊号	1469-8137
出版地	HOBOKEN
WOS关键词	NADPH-PROTOCHLOROPHYLLIDE OXIDOREDUCTASE ; CHLOROPHYLL BIOSYNTHESIS ; POSTTRANSLATIONAL CONTROL ; ARABIDOPSIS ; CHELATASE ; PORPHOBILINOGEN ; IDENTIFICATION ; STABILIZATION ; EMBRYOGENESIS ; EXPRESSION
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	WILEY
WOS记录号	WOS:000819564400001
资助机构	National Key R&D Program of China [2019YFA0904600, 2020YFA0907600] ; National Natural Science Foundation of China [31870213] ; Strategic Priority Research Program of the Chinese Academy of Sciences [XDB27030205] ; Chinese Scholarship Council ; Deutsche Forschungsgemeinschaft [SFB TRR175]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/28529]
专题	中科院光生物学重点实验室
作者单位	1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Humboldt Univ, Inst Biol Plant Physiol, D-10099 Berlin, Germany 3.Chinese Acad Sci, Key Lab Photobiol, Inst Bot, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Yuan, Jiarui,Ma, Tingting,Ji, Shuiling,et al. Two chloroplast-localized MORF proteins act as chaperones to maintain tetrapyrrole biosynthesis[J]. NEW PHYTOLOGIST,2022,235(5):1868-1883.
APA	Yuan, Jiarui,Ma, Tingting,Ji, Shuiling,Hedtke, Boris,Grimm, Bernhard,&Lin, Rongcheng.(2022).Two chloroplast-localized MORF proteins act as chaperones to maintain tetrapyrrole biosynthesis.NEW PHYTOLOGIST,235(5),1868-1883.
MLA	Yuan, Jiarui,et al."Two chloroplast-localized MORF proteins act as chaperones to maintain tetrapyrrole biosynthesis".NEW PHYTOLOGIST 235.5(2022):1868-1883.