An endoplasmic reticulum-associated degradation-related E2-E3 enzyme pair controls grain size and weight through the brassinosteroid signaling pathway in rice

doi:10.1093/plcell/koac364

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	An endoplasmic reticulum-associated degradation-related E2-E3 enzyme pair controls grain size and weight through the brassinosteroid signaling pathway in rice
	Li, Jing 3; Zhang, Baolan 3; Duan, Penggen 3; Yan, Li 3; Yu, Haiyue 3; Zhang, Limin 3; Li, Na 3; Zheng, Leiying 2; Chai, Tuanyao ; Xu, Ran 1,6
刊名	PLANT CELL
	2023
卷号	35 期号:3 页码:1076-1091
ISSN号	1040-4651
DOI	10.1093/plcell/koac364
文献子类	Article
英文摘要	Grain size is an important agronomic trait, but our knowledge about grain size determination in crops is still limited. Endoplasmic reticulum (ER)-associated degradation (ERAD) is a special ubiquitin proteasome system that is involved in degrading misfolded or incompletely folded proteins in the ER. Here, we report that SMALL GRAIN 3 (SMG3) and DECREASED GRAIN SIZE 1 (DGS1), an ERAD-related E2-E3 enzyme pair, regulate grain size and weight through the brassinosteroid (BR) signaling pathway in rice (Oryza sativa). SMG3 encodes a homolog of Arabidopsis (Arabidopsis thaliana) UBIQUITIN CONJUGATING ENZYME 32, which is a conserved ERAD-associated E2 ubiquitin conjugating enzyme. SMG3 interacts with another grain size regulator, DGS1. Loss of function of SMG3 or DGS1 results in small grains, while overexpression of SMG3 or DGS1 leads to long grains. Further analyses showed that DGS1 is an active E3 ubiquitin ligase and colocates with SMG3 in the ER. SMG3 and DGS1 are involved in BR signaling. DGS1 ubiquitinates the BR receptor BRASSINOSTEROID INSENSITIVE 1 (BRI1) and affects its accumulation. Genetic analysis suggests that SMG3, DGS1, and BRI1 act together to regulate grain size and weight. In summary, our findings identify an ERAD-related E2-E3 pair that regulates grain size and weight, which gives insight into the function of ERAD in grain size control and BR signaling. An endoplasmic reticulum-associated degradation-related E2-E3 pair affects grain size in rice through the brassinosteroid signaling pathway.
学科主题	Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology
电子版国际标准刊号	1532-298X
出版地	CARY
WOS关键词	ACTIVATED PROTEIN-KINASE ; NATURAL VARIATION ; QUALITY-CONTROL ; OSMKKK10-OSMKK4-OSMPK6 CASCADE ; TRANSCRIPTION FACTORS ; ENCODES ; BIOMASS ; PLAYS ; COMPONENT ; NUMBER
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	OXFORD UNIV PRESS INC
WOS记录号	WOS:000924809700001
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/29073]
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 2.Chinese Acad Sci, Inst Genet & Dev Biol, CAS Ctr Excellence Mol Plant Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China 3.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China 4.Hainan Univ, Coll Trop Crops, Haikou 570288, Peoples R China 5.Chinese Acad Sci, Innovat Seed Design, Sanya 572025, Peoples R China 6.Hainan Univ, Sanya Nanfan Res Inst, Hainan Yazhou Bay Seed Lab, Sanya 572025, Peoples R China
推荐引用方式 GB/T 7714	Li, Jing,Zhang, Baolan,Duan, Penggen,et al. An endoplasmic reticulum-associated degradation-related E2-E3 enzyme pair controls grain size and weight through the brassinosteroid signaling pathway in rice[J]. PLANT CELL,2023,35(3):1076-1091.
APA	Li, Jing.,Zhang, Baolan.,Duan, Penggen.,Yan, Li.,Yu, Haiyue.,...&Li, Yunhai.(2023).An endoplasmic reticulum-associated degradation-related E2-E3 enzyme pair controls grain size and weight through the brassinosteroid signaling pathway in rice.PLANT CELL,35(3),1076-1091.
MLA	Li, Jing,et al."An endoplasmic reticulum-associated degradation-related E2-E3 enzyme pair controls grain size and weight through the brassinosteroid signaling pathway in rice".PLANT CELL 35.3(2023):1076-1091.