The discovery of a key prenyltransferase gene assisted by a chromosome-level Epimedium pubescens genome

doi:10.3389/fpls.2022.1034943

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院植物分子生理学重点实验室

	The discovery of a key prenyltransferase gene assisted by a chromosome-level Epimedium pubescens genome
	Shen, Guoan ; Luo, Yanjiao 6; Yao, Yu ; Meng, Guoqing 1,5; Zhang, Yixin ; Wang, Yuanyue ; Xu, Chaoqun ; Liu, Xiang 4; Zhang, Cheng 3; Ding, Gang
刊名	FRONTIERS IN PLANT SCIENCE
	2022
卷号	13
关键词	Epimedium pubescens genome assembly prenyltransferase prenylated flavonoids whole genome duplication
ISSN号	1664-462X
DOI	10.3389/fpls.2022.1034943
文献子类	Article
英文摘要	Epimedium pubescens is a species of the family Berberidaceae in the basal eudicot lineage, and a main plant source for the traditional Chinese medicine Herba Epimedii. The current study achieved a chromosome-level genome assembly of E. pubescens with the genome size of 3.34 Gb, and the genome guided discovery of a key prenyltransferase (PT) in E. pubescens. Our comparative genomic analyses confirmed the absence of Whole Genome Triplication (WGT-gamma) event shared in core eudicots and further revealed the occurrence of an ancient Whole Genome Duplication (WGD) event approximately between 66 and 81 Million Years Ago (MYA). In addition, whole genome search approach was successfully applied to identify 19 potential flavonoid PT genes and an important flavonoid PT (EpPT8) was proven to be an enzyme for the biosynthesis of medicinal compounds, icaritin and its derivatives in E. pubescens. Therefore, our results not only provide a good reference genome to conduct further molecular biological studies in Epimedium genus, but also give important clues for synthetic biology and industrial production of related prenylated flavonoids in future.
学科主题	Plant Sciences
出版地	LAUSANNE
WOS关键词	FLAVONOIDS ; BERBERIDACEAE ; CONTRIBUTES ; DIVERGENCE ; INSIGHTS ; ICARITIN ; SIZE
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	FRONTIERS MEDIA SA
WOS记录号	WOS:000890407300001
资助机构	CAMS Innovation Fund for Medical Sciences (CIFMS) ; National Natural Science Foundation of China ; Chongqing Science and Technology Commission ; [2021-I2M-1-031] ; [2017-I2M-3-013] ; [31570306] ; [U20A2004] ; [81473302] ; [cstc2018jcyjAX0316] ; [cc-cstc-CA-19-2] ; [cstc2019jcyj-msxmx0464] ; [cstc2019jxjl-jbky10007] ; [cstc2020jxjl10004]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/29040]
专题	中科院植物分子生理学重点实验室
作者单位	1.Chinese Acad Agr Sci, Inst Anim Sci, Beijing, Peoples R China 2.Beijing Normal Univ, Coll Life Sci, Key Lab Biodivers Sci & Ecol Engn, Minist Educ, Beijing, Peoples R China 3.Chongqing Acad Chinese Mat Med, Chongqing Key Lab Tradit Chinese Med Resource, Chongqing, Peoples R China 4.Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China 5.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing, Peoples R China 6.Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Plant Dev, Key Lab Bioact Subst & Resources Utilizat Chinese, Minist Educ, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Shen, Guoan,Luo, Yanjiao,Yao, Yu,et al. The discovery of a key prenyltransferase gene assisted by a chromosome-level Epimedium pubescens genome[J]. FRONTIERS IN PLANT SCIENCE,2022,13.
APA	Shen, Guoan.,Luo, Yanjiao.,Yao, Yu.,Meng, Guoqing.,Zhang, Yixin.,...&Guo, Baolin.(2022).The discovery of a key prenyltransferase gene assisted by a chromosome-level Epimedium pubescens genome.FRONTIERS IN PLANT SCIENCE,13.
MLA	Shen, Guoan,et al."The discovery of a key prenyltransferase gene assisted by a chromosome-level Epimedium pubescens genome".FRONTIERS IN PLANT SCIENCE 13(2022).