Oxidosqualene Cyclases Involved in the Biosynthesis of Diverse Triterpenes in Camellia sasanqua

doi:10.1021/acs.jafc.2c03011

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	Oxidosqualene Cyclases Involved in the Biosynthesis of Diverse Triterpenes in Camellia sasanqua
	Huang, Liufang ; Hu, Yonger ; Huang, Ruoshi ; Chen, Jiabo ; Zhang, Xiande ; Yue, Jingyang ; Feng, Laibao 3; She, Yaru ; Ji, Aijia ; Zheng, Ying 2
刊名	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
	2022
关键词	oxidosqualene cyclases (OSCs) Camellia sasanqua triterpene biosynthesis catalytic mechanism
ISSN号	0021-8561
DOI	10.1021/acs.jafc.2c03011
文献子类	Article; Early Access
英文摘要	Camellia sasanqua is an important economic plant that is rich in lipophilic triterpenols with pharmacological activities including antiallergic, anti-inflammatory, and anticancer activities. However, the key enzymes related to triterpene biosynthesis have seldom been studied in C. sasanqua. Oxidosqualene cyclases (OSCs) are the rate-limiting enzymes related to triterpene biosynthesis. In this study, seven putative OSC genes (CsOSC1-7) were mined from the C. sasanqua transcriptome. Six CsOSCs were characterized for the biosynthesis of diverse triterpene skeletons, including alpha-amyrin, beta-amyrin, delta-amyrin, dammarenediol-II, psi taraxasterol, taraxasterol, and cycloartenol by the heterologous expression system. CsOSC3 was a multiple functional alpha-amyrin synthase. Three key residues, Trp260, Tyr262, and Phe415, are critical to the catalytic performance of CsOSC3 judging from the results of molecular docking and site-directed mutagenesis. These findings provide important insights into the biosynthesis pathway of triterpenes in C. sasanqua.
学科主题	Agriculture, Multidisciplinary ; Chemistry, Applied ; Food Science & Technology
电子版国际标准刊号	1520-5118
出版地	WASHINGTON
WOS关键词	FLOWER BUDS ; SYNTHASE ; ALCOHOLS ; EXPRESSION ; PLANT ; OILS
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	AMER CHEMICAL SOC
WOS记录号	WOS:000821821000001
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/28807]
专题	中科院植物分子生理学重点实验室
作者单位	1.Guangzhou Univ Chinese Med, Basic Med Sci Coll, Res Ctr Basic Integrat Med, Guangzhou 510006, Peoples R China 2.Chinese Acad Sci, Inst Bot, Key Lab Plant Mol Physiol, Beijing 100093, Peoples R China 3.Guangzhou Univ Chinese Med, Sch Pharmaceut Sci, Int Inst Translat Chinese Med, Joint Lab Translat Canc Res Chinese Med,Minist Ed, Guangzhou 510006, Peoples R China
推荐引用方式 GB/T 7714	Huang, Liufang,Hu, Yonger,Huang, Ruoshi,et al. Oxidosqualene Cyclases Involved in the Biosynthesis of Diverse Triterpenes in Camellia sasanqua[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2022.
APA	Huang, Liufang.,Hu, Yonger.,Huang, Ruoshi.,Chen, Jiabo.,Zhang, Xiande.,...&Duan, Lixin.(2022).Oxidosqualene Cyclases Involved in the Biosynthesis of Diverse Triterpenes in Camellia sasanqua.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY.
MLA	Huang, Liufang,et al."Oxidosqualene Cyclases Involved in the Biosynthesis of Diverse Triterpenes in Camellia sasanqua".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2022).