Rice Cell Division Cycle 20s are required for faithful chromosome segregation and cytokinesis during meiosis

doi:10.1093/plphys/kiab543

CORC > 植物研究所 > 中国科学院植物研究所 > 中科院植物分子生理学重点实验室

	Rice Cell Division Cycle 20s are required for faithful chromosome segregation and cytokinesis during meiosis
	Lin, Ya-Nan ; Jiang, Chen-Kun ; Cheng, Zhu-Kuan ; Wang, Dong-Hui ; Shen, Li-Ping ; Xu, Cong ; Xu, Zhi-Hong ; Bai, Shu-Nong
刊名	PLANT PHYSIOLOGY
	2022
卷号	188 期号:2 页码:1111-1128
ISSN号	0032-0889
DOI	10.1093/plphys/kiab543
文献子类	Article
英文摘要	Chromosome segregation must be under strict regulation to maintain chromosome euploidy and stability. Cell Division Cycle 20 (CDC20) is an essential cell cycle regulator that promotes the metaphase-to-anaphase transition and functions in the spindle assembly checkpoint, a surveillance pathway that ensures the fidelity of chromosome segregation. Plant CDC20 genes are present in multiple copies, and whether CDC20s have the same functions in plants as in yeast and animals is unclear, given the potential for divergence or redundancy among the multiple copies. Here, we studied all three CDC20 genes in rice (Oryza sativa) and constructed two triple mutants by clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9-mediated genome editing to explore their roles in development. Knocking out all three CDC20 genes led to total sterility but did not affect vegetative development. Loss of the three CDC20 proteins did not alter mitotic division but severely disrupted meiosis as a result of asynchronous and unequal chromosome segregation, chromosome lagging, and premature separation of chromatids. Immunofluorescence of tubulin revealed malformed meiotic spindles in microsporocytes of the triple mutants. Furthermore, cytokinesis of meiosis I was absent or abnormal, and cytokinesis II was completely prevented in all mutant microsporocytes; thus, no tetrads or pollen formed in either cdc20 triple mutant. Finally, the subcellular structures and functions of the tapetum were disturbed by the lack of CDC20 proteins. These findings demonstrate that the three rice CDC20s play redundant roles but are indispensable for faithful meiotic chromosome segregation and cytokinesis, which are required for the production of fertile microspores. Loss of all three rice Cell Division Cycle 20 genes caused male sterility, meiotic chromosome missegregation, aberrant spindle morphology, loss of meiotic cytokinesis, and disrupted tapetal function.
学科主题	Plant Sciences
电子版国际标准刊号	1532-2548
出版地	CARY
WOS关键词	SPINDLE-ASSEMBLY CHECKPOINT ; SISTER-CHROMATID COHESION ; CENTROMERIC COHESION ; METAPHASE ARREST ; PROTEIN ; CDC20 ; TAPETUM ; ARABIDOPSIS ; COMPLEX ; SHUGOSHIN
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	OXFORD UNIV PRESS INC
WOS记录号	WOS:000761476800040
资助机构	Ministry of Agriculture of the People's Republic of China [2016ZX08010001, 2016ZX08009-003] ; National Natural Science Foundation of China [31630006]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/28474]
专题	中科院植物分子生理学重点实验室
作者单位	1.Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China 2.[Lin, Ya-Nan 3.Wang, Dong-Hui 4.Xu, Cong 5.Xu, Zhi-Hong 6.State Key Lab Prot & Plant Gene Res, Beijing 100871, Peoples R China 7.Jiang, Chen-Kun 8.Chinese Acad Sci, State Key Lab Plant Genom, Beijing 100101, Peoples R China 9.Chinese Acad Sci, Ctr Plant Gene Res, Inst Genet & Dev Biol, Beijing 100101, Peoples R China 10.Peking Univ, Natl Teaching Ctr Expt Biol, Beijing 100871, Peoples R China
推荐引用方式 GB/T 7714	Lin, Ya-Nan,Jiang, Chen-Kun,Cheng, Zhu-Kuan,et al. Rice Cell Division Cycle 20s are required for faithful chromosome segregation and cytokinesis during meiosis[J]. PLANT PHYSIOLOGY,2022,188(2):1111-1128.
APA	Lin, Ya-Nan.,Jiang, Chen-Kun.,Cheng, Zhu-Kuan.,Wang, Dong-Hui.,Shen, Li-Ping.,...&Bai, Shu-Nong.(2022).Rice Cell Division Cycle 20s are required for faithful chromosome segregation and cytokinesis during meiosis.PLANT PHYSIOLOGY,188(2),1111-1128.
MLA	Lin, Ya-Nan,et al."Rice Cell Division Cycle 20s are required for faithful chromosome segregation and cytokinesis during meiosis".PLANT PHYSIOLOGY 188.2(2022):1111-1128.