Genome-Resolved Metagenomics Reveals Distinct Phosphorus Acquisition Strategies between Soil Microbiomes

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	Genome-Resolved Metagenomics Reveals Distinct Phosphorus Acquisition Strategies between Soil Microbiomes
	Wu, Xingjie 1; Rensing, Christopher 5; Han, Dongfei 4; Xiao, Ke-Qing ; Dai, Yuexiu 1; Tang, Zhixi 6; Liesack, Werner 3; Peng, Jingjing 1; Cui, Zhenling 1; Zhang, Fusuo 1
刊名	MSYSTEMS
	2022
卷号	7 期号:1
关键词	phosphorus gcd genome microbiome metagenomics MAGs phosphorous
ISSN号	2379-5077
文献子类	Article
英文摘要	Enhancing soil phosphate solubilization is a promising strategy for agricultural sustainability, while little is known about the mechanisms of how microorganisms cope with differing phosphorus availability. Using a combination of genome-resolved metagenomics and amplicon sequencing, we investigated the microbial mechanisms involved in phosphorus cycling under three agricultural treatments in a wheat-maize rotation system and two natural reforestation treatments. Available soil phosphorus was the key factor shaping bacterial and fungal community composition and function across our agricultural and reforestation sites. Membrane-bound quinoprotein glucose dehydrogenase (PQQGDH) and exopolyphosphatases (PPX) governed microbial phosphate solubilization in agroecosystems. In contrast, genes encoding glycerol-3-phosphate transporters (ugpB, ugpC, and ugpQ) displayed a significantly greater abundance in the reforestation soils. The gcd gene encoding PQQGDH was found to be the best determinant for bioavailable soil phosphorus. Metagenome-assembled genomes (MAGs) affiliated with Cyclobacteriaceae and Vicinamibacterales were obtained from agricultural soils. Their MAGs harbored not only gcd but also the pit gene encoding low-affinity phos-phate transporters. MAGs obtained from reforestation soils were affiliated with Microtrichales and Burkholderiales. These contain ugp genes but no gcd, and thereby are indicative of a phosphate transporter strategy. Our study demonstrates that knowledge of distinct microbial phosphorus acquisition strategies between agricultural and reforestation soils could help in linking microbial processes with phosphorus cycling. IMPORTANCE The soil microbiome is the key player regulating phosphorus cycling proc-esses. Identifying phosphate-solubilizing bacteria and utilizing them for release of recal-citrant phosphate that is bound to rocks or minerals have implications for improving crop nutrient acquisition and crop productivity. In this study, we combined functional metagenomics and amplicon sequencing to analyze microbial phosphorus cycling proc-esses in natural reforestation and agricultural soils. We found that the phosphorus ac-quisition strategies significantly differed between these two ecosystems. A microbial phosphorus solubilization strategy dominated in the agricultural soils, while a microbial phosphate transporter strategy was observed in the reforestation soils. We further iden-tified microbial taxa that contributed to enhanced phosphate solubilization in the agro-ecosystem. These microbes are predicted to be beneficial for the increase in phosphate bioavailability through agricultural practices.
学科主题	Microbiology
出版地	WASHINGTON
WOS关键词	PHOSPHATE SOLUBILIZATION ; FERTILIZATION ; GENETICS ; SEQUENCE ; NITROGEN ; PLANT
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	AMER SOC MICROBIOLOGY
WOS记录号	WOS:000760768900010
资助机构	Research and Application of Key Technologies for Soil Quality Improvement and Agricultural Green Development [Z191100004019013] ; National Key Research and Development Program of China [2021YFD1900100] ; National Natural Science Foundation of China [41977038] ; 2115 Talent Development Program of China Agricultural University
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/28728]
专题	植被与环境变化国家重点实验室
作者单位	1.China Agr Univ, Natl Acad Agr Green Dev, Coll Resources & Environm Sci, Key Lab Plant Soil Interact, Beijing, Peoples R China 2.Max Planck Inst Terr Microbiol, Res Grp Methanotroph Bacteria & Environm Genom Tr, Marburg, Germany 3.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing, Peoples R China 4.Fujian Agr & Forestry Univ, Coll Resource & Environm, Inst Environm Microbiol, Fuzhou, Peoples R China 5.Natl Observat & Res Stn Agr Green Dev, Quzhou, Hebei, Peoples R China 6.Univ Leeds, Sch Earth & Environm, Leeds, W Yorkshire, England 7.Chinese Acad Agr Sci, Inst Environm & Sustainable Dev Agr, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wu, Xingjie,Rensing, Christopher,Han, Dongfei,et al. Genome-Resolved Metagenomics Reveals Distinct Phosphorus Acquisition Strategies between Soil Microbiomes[J]. MSYSTEMS,2022,7(1).
APA	Wu, Xingjie.,Rensing, Christopher.,Han, Dongfei.,Xiao, Ke-Qing.,Dai, Yuexiu.,...&Zhang, Fusuo.(2022).Genome-Resolved Metagenomics Reveals Distinct Phosphorus Acquisition Strategies between Soil Microbiomes.MSYSTEMS,7(1).
MLA	Wu, Xingjie,et al."Genome-Resolved Metagenomics Reveals Distinct Phosphorus Acquisition Strategies between Soil Microbiomes".MSYSTEMS 7.1(2022).