Genome-wide analysis of the Glutathione S-Transferase family in wild Medicago ruthenica and drought-tolerant breeding application of MruGSTU39 gene in cultivated alfalfa

doi:10.1007/s00122-021-04002-x

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	Genome-wide analysis of the Glutathione S-Transferase family in wild Medicago ruthenica and drought-tolerant breeding application of MruGSTU39 gene in cultivated alfalfa
	Wang, Tianzuo ; Zhang, Di ; Chen, Li ; Wang, Jing ; Zhang, Wen-Hao
刊名	THEORETICAL AND APPLIED GENETICS
	2022
卷号	135 期号:3 页码:853-864
ISSN号	0040-5752
DOI	10.1007/s00122-021-04002-x
文献子类	Article
英文摘要	Key message Transformation ofMruGSTU39inM. ruthenicaand alfalfa enhanced growth and survival of transgenic plants by up-regulating GST and glutathione peroxidase activity to detoxify ROS under drought stress. Glutathione S-transferases (GSTs) are ubiquitous supergene family which play crucial roles in detoxification of reactive oxygen species (ROS). Despite studies on GSTs, few studies have focused on them in perennial, wild plant species with high tolerance to environmental stress. Here, we identified 66 MruGST genes from the genome of Medicago ruthenica, a perennial legume species native to temperate grasslands with high tolerance to environmental stress. These genes were divided into eight classes based on their conserved domains, phylogenetic tree and gene structure, with the tau class being the most numerous. Duplication analysis revealed that GST family in M. ruthenica was expanded by segmental and tandem duplication. Several drought-responsive MruGSTs were identified by transcriptomic analyses. Of them, expression of MruGSTU39 was up-regulated much more in a tolerant accession by drought stress. Transformation of MruGSTU39 in M. ruthenica and alfalfa (Medicago sativa) enhanced growth and survival of transgenic seedlings than their wild-type counterparts under drought. We demonstrated that MruGSTU39 can detoxify ROS to reduce its damage to membrane by up-regulating activities of GST and glutathione peroxidase. Our findings provide full-scale knowledge on GST family in the wild legume M. ruthenica with high tolerance to drought, and highlight improvement tolerance of legume forages to drought using genomic information of M. ruthenica.
学科主题	Agronomy ; Plant Sciences ; Genetics & Heredity ; Horticulture
电子版国际标准刊号	1432-2242
出版地	NEW YORK
WOS关键词	SEQUENCE ; STRESS ; RADIATION ; OVEREXPRESSION ; SENSITIVITY ; REVEALS ; BARLEY ; GROWTH
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000722091100001
资助机构	National Natural Science Foundation of China [32070351, 31830011] ; Science and Technology Program of Inner Mongolia, China [2021GG0372]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/28676]
专题	植被与环境变化国家重点实验室
作者单位	1.Wang, Jing 2.Chen, Li 3.Zhang, Di 4.[Wang, Tianzuo 5.Univ Chinese Acad Sci, Chinese Acad Sci, Coll Resources & Environm, Beijing, Peoples R China 6.Chinese Acad Sci, Inst Bot, State Key Lab Vegetat & Environm Change, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Wang, Tianzuo,Zhang, Di,Chen, Li,et al. Genome-wide analysis of the Glutathione S-Transferase family in wild Medicago ruthenica and drought-tolerant breeding application of MruGSTU39 gene in cultivated alfalfa[J]. THEORETICAL AND APPLIED GENETICS,2022,135(3):853-864.
APA	Wang, Tianzuo,Zhang, Di,Chen, Li,Wang, Jing,&Zhang, Wen-Hao.(2022).Genome-wide analysis of the Glutathione S-Transferase family in wild Medicago ruthenica and drought-tolerant breeding application of MruGSTU39 gene in cultivated alfalfa.THEORETICAL AND APPLIED GENETICS,135(3),853-864.
MLA	Wang, Tianzuo,et al."Genome-wide analysis of the Glutathione S-Transferase family in wild Medicago ruthenica and drought-tolerant breeding application of MruGSTU39 gene in cultivated alfalfa".THEORETICAL AND APPLIED GENETICS 135.3(2022):853-864.