Natural lupeol level variation among castor accessions and the upregulation of lupeol synthesis in response to light

doi:10.1016/j.indcrop.2022.116090

CORC > 植物研究所 > 中国科学院植物研究所 > 系统与进化植物学国家重点实验室

	Natural lupeol level variation among castor accessions and the upregulation of lupeol synthesis in response to light
	Li, Donghai 1,7; Zaman, Wajid 2; Lu, Jianjun 1; Niu, Qingqing ; Zhang, Xuanhao ; Ayaz, Asma ; Saqib, Saddam 3; Yang, Baiming 4; Zhang, Jixing 5; Zhao, Huayan
刊名	INDUSTRIAL CROPS AND PRODUCTS
	2023
卷号	192
关键词	Epidermal wax Castor varieties Lupeol synthesis RcLUS-1 Gene regulation
ISSN号	0926-6690
DOI	10.1016/j.indcrop.2022.116090
文献子类	Article
英文摘要	Lupeol, a plant-derived lupane-type pentacyclic triterpenoid, has many pharmacological activities. Although lupeol is found widely in many fruits, vegetables, and medicinal plants, its low content and difficult extraction limit its current applications. The castor plant (Ricinus communis L.) is a potential candidate plant for producing lupeol because it accumulates at high levels as a dominant wax component in the epidermal surface. The lupeol content varies significantly among castor individuals/cultivars. To identify castor lines for further breeding and uncover the molecular mechanism of lupeol biosynthesis and regulation, several castor varieties with various lupeol levels were identified, including 26 lines with higher lupeol contents and 12 glossy lines with very low lupeol contents. A deletion of the coding sequence of castor lupeol synthase RcLUS-1 was identified in glossy1, which possibly affects the binding ability of the substrate. The role of RcLUS-1 in lupeol production was determined using a virus-induced gene silencing (VIGS) assay. The promoter region of RcLUS-1 was analyzed further to uncover the factors affecting lupeol synthesis. Several potential cis-acting elements responding to light present were enriched in this region. Further study showed that light intensity affects RcLUS-1 expression and lupeol production. Overall, this study evaluated several potential castor resources for future breeding and expanded the knowledge of the lupeol regulation mechanism.
学科主题	Agricultural Engineering ; Agronomy
电子版国际标准刊号	1872-633X
出版地	AMSTERDAM
WOS关键词	OXIDOSQUALENE CYCLASE ; MESSENGER-RNAS ; TRITERPENE ; REQUIREMENT ; EXPRESSION ; SYNTHASE ; DUCKWEED ; ELEMENT ; CLONING
WOS研究方向	Science Citation Index Expanded (SCI-EXPANDED)
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:000934011300001
资助机构	Natural Science Foundation of China [31760399]
内容类型	期刊论文
源URL	[http://ir.ibcas.ac.cn/handle/2S10CLM1/29147]
专题	系统与进化植物学国家重点实验室
作者单位	1.Hubei Hongshan Lab, Wuhan 430070, Peoples R China 2.Chinese Acad Sci, CAS Key Lab Plant Germplasm Enhancement & Special, Wuhan Bot Garden, Wuhan 430074, Peoples R China 3.Yeungnam Univ, Dept Life Sci, Gyongsan 38541, Gyeongbuk, South Korea 4.Chinese Acad Sci, Inst Bot, State Key Lab Systemat & Evolutionary Bot, Beijing 100093, Peoples R China 5.Jilin Agr Sci & Technol Univ, Jilin 132109, Jilin, Peoples R China 6.Inner Mongolia Univ Nationalities, Coll Life Sci & Food Engn, Tongliao 028000, Peoples R China 7.Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Wuhan 430062, Peoples R China
推荐引用方式 GB/T 7714	Li, Donghai,Zaman, Wajid,Lu, Jianjun,et al. Natural lupeol level variation among castor accessions and the upregulation of lupeol synthesis in response to light[J]. INDUSTRIAL CROPS AND PRODUCTS,2023,192.
APA	Li, Donghai.,Zaman, Wajid.,Lu, Jianjun.,Niu, Qingqing.,Zhang, Xuanhao.,...&Lu, Shiyou.(2023).Natural lupeol level variation among castor accessions and the upregulation of lupeol synthesis in response to light.INDUSTRIAL CROPS AND PRODUCTS,192.
MLA	Li, Donghai,et al."Natural lupeol level variation among castor accessions and the upregulation of lupeol synthesis in response to light".INDUSTRIAL CROPS AND PRODUCTS 192(2023).