Cloning and functional characterization of the oxidative squalene cyclase gene in the deep-sea holothurian Chiridota sp.

doi:10.1016/j.gene.2023.147971

CORC > 深海科学与工程研究所 > 中国科学院深海科学与工程研究所 > 深海科学研究部 > 深海生物学研究室 > 海洋生物生态与进化研究组

	Cloning and functional characterization of the oxidative squalene cyclase gene in the deep-sea holothurian Chiridota sp.
	Huang, Yanan 2,3; Liu, Helu 3; Zhou, Yang 3; Lu, Zaiqing 1; Pu, Yujin 2,3; Zhang, Haibin 3
刊名	GENE
	2024-02-05
卷号	894 页码:9
关键词	Deep-sea Holothurian Chiridota sp Sea cucumber triterpenes GC -MS
ISSN号	0378-1119
DOI	10.1016/j.gene.2023.147971
通讯作者	Zhang, Haibin(hzhang@idsse.ac.cn)
英文摘要	Saponins derived from holothurians have high potential medicinal value. However, the de novo synthesis of the derivatization of triterpenes is still unclear. Oxidative squalene cyclase (OSC) can catalyze 2,3-Oxidosqualene into diverse products that serve as important precursors for triterpene synthesis. However, the function of the OSC gene in Chiridota sp. has not been elucidated. In this study, an OSC gene derived from the deep-sea holothurian Chiridota sp. was cloned and characterized functionally in a yeast system. The open reading frame of the OSC gene was 2086 bp, which encoded 695 amino acids. The Chiridota sp. OSC gene has a similarity of 66.89 % to the OSC of other holothurian species and 63.51 % to that of Acanthaster planci. The phylogenetic tree showed that the echinozoan OSCs clustered together, and then they formed a sister group to fungi and plant homologs. Chiridota sp. OSC catalyzed 2,3-Oxidosqualene into parkeol. Under high pressure, the relative enzymatic activity and stability of cyclase in Chiridota sp. was higher than that in the shallow-sea holothurian Stichopus horrens. The newly cloned OSC of Chiridota sp. provides key information for the interpretation of the saponin synthesis pathway in deep-sea holothurians.
资助项目	Major scientific and technological projects of Hainan Province[ZDKJ2021036] ; Strategic Priority Research Program of the Chinese Academy of Sciences (CAS)[XDA22050303] ; National Key Research and Development Program of China[2016YFC0304905]
WOS关键词	BETA-AMYRIN SYNTHASE ; HIGH-PRESSURE ; TRITERPENE GLYCOSIDES ; OXIDOSQUALENE CYCLASE ; BIOSYNTHESIS ; SAPONINS ; CYCLOARTENOL ; EXPRESSION ; LANOSTEROL
WOS研究方向	Genetics & Heredity
语种	英语
出版者	ELSEVIER
WOS记录号	WOS:001119752000001
资助机构	Major scientific and technological projects of Hainan Province ; Strategic Priority Research Program of the Chinese Academy of Sciences (CAS) ; National Key Research and Development Program of China
内容类型	期刊论文
源URL	[http://ir.idsse.ac.cn/handle/183446/10712]
专题	深海科学研究部_深海生物学研究室_海洋生物生态与进化研究组
通讯作者	Zhang, Haibin
作者单位	1.Ocean Univ China, Qingdao 266100, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Inst Deep sea Sci & Engn, Sanya 572000, Peoples R China
推荐引用方式 GB/T 7714	Huang, Yanan,Liu, Helu,Zhou, Yang,et al. Cloning and functional characterization of the oxidative squalene cyclase gene in the deep-sea holothurian Chiridota sp.[J]. GENE,2024,894:9.
APA	Huang, Yanan,Liu, Helu,Zhou, Yang,Lu, Zaiqing,Pu, Yujin,&Zhang, Haibin.(2024).Cloning and functional characterization of the oxidative squalene cyclase gene in the deep-sea holothurian Chiridota sp..GENE,894,9.
MLA	Huang, Yanan,et al."Cloning and functional characterization of the oxidative squalene cyclase gene in the deep-sea holothurian Chiridota sp.".GENE 894(2024):9.